Lau, Y.L. and Shamilah, H. and Fong, M.Y. (2006) Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. Tropical Biomedicine, 23 (2). pp. 186-93. ISSN 0127-5720, DOI 17322821.
Full text not available from this repository.Abstract
A truncated form of surface antigen 2 (SAG2) of the protozoan parasite Toxoplasma gondii was cloned and expressed in the methylotrophic yeast Pichia pastoris. This recombinant antigen, designated as recSAG2-N, contained only the N-terminal half of the native SAG2. The recSAG2-N was secreted by the Pichia pastoris into the culture supernatant, and it was harvested by using the trichloroacetic acid precipitation method. Specificity of recSAG2-N was evaluated in western blot assays. Fifty human serum samples, including 32 from confirmed cases of toxoplasmosis, were tested. Results from the assays showed that recSAG2-N reacted with sera from the toxoplasmosis cases only. In vivo experiments showed that serum from mice which received recSAG2-N reacted with the native SAG2 of T. gondii.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Department of Parasitology, Faculty of Medicine, University of Malaya |
| Uncontrolled Keywords: | Amino Acid Sequence; Animals; Antibodies, Protozoan/blood; |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Medicine |
| Depositing User: | Mr. Faizal Hamzah |
| Date Deposited: | 24 Mar 2011 04:31 |
| Last Modified: | 21 Oct 2014 04:37 |
| URI: | http://eprints.um.edu.my/id/eprint/622 |
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