Vijayarathna, S. and Zakaria, Z. and Chen, Y. and Latha, L.Y. and Kanwar, J.R. and Sasidharan, S. (2012) The antimicrobial efficacy of elaeis guineensis: characterization, in vitro and in vivo studies. Molecules, 17 (5). pp. 4860-4877. ISSN 1420-3049, DOI https://doi.org/10.3390/molecules17054860.
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Abstract
The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity in vitro and in vivo against the bacterial and fungal strains tested. A marked inhibitory effect of the E. guineensis extracts was observed against C. albicans whereby E. guineensis extract at 1/2, 1, or 2 times the MIC significantly inhibited C. albicans growth with a noticeable drop in optical density (OD) of the bacterial culture. This finding confirmed the anticandidal activity of the extract on C. albicans. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated C. albicans. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. In vivo antimicrobial activity was studied in mice that had been inoculated with C. albicans and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.
| Item Type: | Article |
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| Funders: | UNSPECIFIED |
| Additional Information: | ISI Document Delivery No.: 949PH Times Cited: 0 Cited Reference Count: 42 Cited References: Abubakar EMM, 2009, J MED PLANTS RES, V3, P498 Alzoreky N.S., 2003, INT J FOOD MICROBIOL, V80, P23 Amato-Gauci A., 2007, 1 EUROPEAN COMMUNICA ANAISSIE E, 1993, INFECT IMMUN, V61, P1268 Bauer A.W., 1996, AM J CLIN PATHOL, V36, P493 BELAY G, 2011, PHYTOPHARMACOLOGY, V1, P166 BORGERS M, 1989, J MED VET MYCOL, V27, P381 Boucher HW, 2009, CLIN INFECT DIS, V48, P1, DOI 10.1086/595011 Bradley JS, 2007, LANCET INFECT DIS, V7, P68, DOI 10.1016/S1473-3099(06)70689-2 Cars O., 2008, BRIT MED J, V337, P1438 Chong K.H., 2008, PHARMACOLOGYONLINE, V3, P379 Cowan MM, 1999, CLIN MICROBIOL REV, V12, P564 Dongmo A.B., 1998, BR J NUTR, V80, P77 Fattouch S, 2007, J AGR FOOD CHEM, V55, P963, DOI 10.1021/jf062614e GEISSMAN T A, 1963, PYRROLE PIGMENTS ISO Gopalakrishnan S., 2011, INT J PHARM BIOSCI, V2, P314 IRVIN TT, 1985, ARCH EMERG MED, V2, P3 Jabeen R, 2008, PAK J BOT, V40, P1349 Kabir A.K.M.S., 2005, INT J AGRIC BIOL, V7, P754 Latha LY, 2011, EUR REV MED PHARMACO, V15, P543 Lepape A., 2009, EUROSURVEILLANCE, V14, P1 MARES D, 1989, MYCOPATHOLOGIA, V108, P37, DOI 10.1007/BF00436782 MASON TL, 1987, PHYTOCHEMISTRY, V26, P2197, DOI 10.1016/S0031-9422(00)84683-X NCCLS, 2002, M100S12 NCCLS Nordmann P, 2009, LANCET INFECT DIS, V9, P228, DOI 10.1016/S1473-3099(09)70054-4 PATHAK D, 1991, Fitoterapia, V62, P371 Rajoo A, 2010, AFR J BIOTECHNOL, V9, P7544 RIGAL L, 1983, BIOMASS, V3, P151, DOI 10.1016/0144-4565(83)90003-3 Sasidharan S, 2010, MOLECULES, V15, P3186, DOI 10.3390/molecules15053186 Sasidharan S., 2009, PHARMACOLOGYONLINE, V3, P84 SCALBERT A, 1991, PHYTOCHEMISTRY, V30, P3875, DOI 10.1016/0031-9422(91)83426-L SCHEIN CH, 1990, BIO-TECHNOL, V8, P308, DOI 10.1038/nbt0490-308 Shittu L.A.J., 2007, IRAN J PHARM THER, V6, P165 Soetardjo Surjani Jr, 2007, Malays J Nutr, V13, P81 Sultana T., 2010, BANGLADESH J SCI IND, V4, P27 Syahmi ARM, 2010, MOLECULES, V15, P8111, DOI 10.3390/molecules15118111 TASSOU CC, 1994, J FOOD PROTECT, V57, P120 Tian F, 2009, FOOD CHEM, V113, P173, DOI 10.1016/j.foodchem.2008.07.062 TRANTER HS, 1993, J APPL BACTERIOL, V74, P253, DOI 10.1111/j.1365-2672.1993.tb03023.x URS NVR, 1975, PHYTOPATHOLOGY, V65, P686 Zheng C.J., 2008, J AGR FOOD CHEM, V24, P11752 Zhu X., 2005, J FOOD SCI, V70, P149 Vijayarathna, Soundararajan Zakaria, Zuraini Chen, Yeng Latha, Lachimanan Yoga Kanwar, Jagat R. Sasidharan, Sreenivasan Institute for Postgraduate Studies (IPS) of Universiti Sains Malaysia Vijayarathna is supported by the Graduate Assistant Scheme from Institute for Postgraduate Studies (IPS) of Universiti Sains Malaysia. Mdpi ag Basel |
| Uncontrolled Keywords: | Antimicrobial activity Candida albicans Elaeis guineensis scanning electron microscopy transmission electron microscopy staphylococcus-aureus phenolic-compounds extracts bacteria system model |
| Subjects: | R Medicine > RK Dentistry |
| Divisions: | Faculty of Dentistry |
| Depositing User: | Mr Ahmad Azwan Azman |
| Date Deposited: | 14 May 2013 01:29 |
| Last Modified: | 14 May 2013 01:29 |
| URI: | http://eprints.um.edu.my/id/eprint/6066 |
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