Triploidy induction by heat-shock treatment in red tilapia

Pradeep, P.J. and Srijaya, T.C. and Bahuleyan, A. and Renjithkumar, C.R. and Jose, D. and Papini, A. and Chatterji, A.K. (2012) Triploidy induction by heat-shock treatment in red tilapia. Caryologia, 65 (2). pp. 152-156. ISSN 0008-7114


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The possible use of sterile triploid red tilapia is an interesting option for culture due to their proliferating breeding activities. The aim of the present study was to investigate and optimize the time of heat-shock treatment to prevent second polar body extrusion from the newly fertilized eggs of red tilapia, to produce a maximum number of triploid individuals. Heat-shock treatment was applied at a temperature of 41 degrees C for a total duration of 3.5 minutes after 2, 3, 4, 4.5, 5 and 6 minutes of fertilization. The best survival rate (67.0) and triploid percentage (89.7) was observed for the treatment at 4 minutes after fertilization. It is thus successfully demonstrated that 4 minutes after fertilization was the most suitable timing of heat-shock treatment for second polar body retention in newly fertilized eggs of red tilapia.

Item Type: Article
Additional Information: ISI Document Delivery No.: 028ON Times Cited: 0 Cited Reference Count: 21 Cited References: Chang SL, 1996, ICLARM C P, V41, P273 DON J, 1986, THEOR APPL GENET, V72, P186, DOI 10.1007/BF00266991 DON J, 1988, J FISH BIOL, V32, P665, DOI 10.1111/j.1095-8649.1988.tb05406.x HUNTER GA, 1983, FISH PHYSIOL, V9, P223 Hussain M.G., 1996, ASIAN FISH SCI, V9, P45 HUSSAIN MG, 1991, THEOR APPL GENET, V81, P6 Linhart O, 2001, J APPL ICHTHYOL, V17, P247, DOI 10.1046/j.1439-0426.2001.00299.x LINHART O, 1991, Aquatic Living Resources, V4, P139, DOI 10.1051/alr:1991014 MAIR GC, 1993, AQUACULTURE, V111, P227, DOI 10.1016/0044-8486(93)90040-6 Maxime V, 2008, FISH FISH, V9, P67, DOI 10.1111/j.1467-2979.2007.00269.x Pandian TJ, 1988, ICLARM C P, V15, P243 Papini A, 2011, PROTOPLASMA, V248, P651, DOI 10.1007/s00709-010-0221-x Peruzzi S, 2007, AQUAC RES, V38, P926, DOI 10.1111/j.1365-2109.2007.01742.x Piferrer F, 2009, AQUACULTURE, V293, P125, DOI 10.1016/j.aquaculture.2009.04.036 Pradeep P. J., 2011, Caryologia, V64, P233 Pradeep PJ, 2010, J COASTAL ENV, V1, P91 Pradeep PJ, 2012, AQUACULTURE, V344, P181, DOI 10.1016/j.aquaculture.2012.03.006 Pradeep PJ, 2011, THESIS U MALAYSIA TE, P306 Pradeep PJ, 2011, TILAPIA AQUACULTURE, P143 Puckhaber B, 1996, ICLARM C P, V41, P377 Tiwary BK, 2004, REV FISH BIOL FISHER, V14, P391, DOI 10.1007/s11160-004-8361-8 Pradeep, Padmaja Jayaprasad Srijaya, Thekkeparambil Chandrabose Bahuleyan, Aneesh Renjithkumar, Chelapurath Radhakrishnan Jose, Deepak Papini, Alessio Chatterji, Anil Kumar University Malaysia Terengganu This work was part of the PhD studies of PJP at University Malaysia Terengganu. The authors are grateful to the University Malaysia Terengganu for providing SKS fellowships to PJP, TCS and a Principal Research Fellowship to AKC. The authors are also thankful to two anonymous reviewers for their valuable comments in improving the quality of the present research work. Univ florence botany inst Florence
Uncontrolled Keywords: Polar body red tilapia time after fertilization triploidy fish biology l.
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 08 May 2013 01:52
Last Modified: 08 May 2013 01:52

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