Tan, S.K. and Pippen, R. and Yusof, R. and Ibrahim, H. and Rahman, N. and Khalid, N. (2005) Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis. In Vitro Cellular and Developmental Biology - Plant, 41 (6). pp. 757-761. ISSN 10545476, DOI https://doi.org/10.1079/IVP2005695.
Full text not available from this repository.Abstract
Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/ Kaempferia pandurata (Schult), to ensure a superior and consistent supply of materials for commercialization purposes. In this study, a single-medium formulation of Murashige and Skoog (MS) supplemented with 13.54 μM 2,4-dichlorophenoxyacetic acid (2,4-D) was found to be the only medium out of eight formulations to promote the complete somatic embryogenesis process for the culture of B. rotunda (L.). Callus cultures were initiated from a total of 280 explants of rhizome meristem. The percentage of cultures forming embryogenic callus was 23.3 ± 4.3 on this MS medium augmented by 13.54 μM 2,4-D. The best plantlet regeneration rate was attained from the first subcultured callus with a mean of 6.6 ± 0.1 plantlets per 1 cm diameter aggregate of callus. Somatic embryogenesis characteristic of monocots was evident from histological studies. The regenerated plantlets have been successfully established in soil. © 2005 Society for In Vitro Biology.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Auxins Ginger Micropropagation Zingiberaceae Boesenbergia rotunda Liliopsida Zingiber officinale |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 19 Jan 2013 07:22 |
| Last Modified: | 20 Oct 2014 03:42 |
| URI: | http://eprints.um.edu.my/id/eprint/6010 |
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