Rapid Micropropagation of boesenbergia rotunda (L.) Mansf. kulturpfl. (a Valuable Medicinal Plant) from shoot bud explants

Yusuf, N.A. and Suffian Annuar, M.M. and Khalid, N. (2011) Rapid Micropropagation of boesenbergia rotunda (L.) Mansf. kulturpfl. (a Valuable Medicinal Plant) from shoot bud explants. African Journal of Biotechnology, 10 (7). pp. 1194-1199. ISSN 16845315

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Abstract

A successful protocol was developed for mass propagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl., an important medicinal plant. Numerous shoots were induced from young shoot bud of B. rotunda mature rhizome on Murashige and Skoog (1962) medium supplemented with 30.0 g/l sucrose, 2.0 g/l gelrite, different concentrations of 6-benzylaminopurine (BAP) and α-naphtaleneacetic acid (NAA). Plant medium supplemented with different concentrations of BAP alone or with NAA produced varying degree of multiple shoots. A supplementation of 2.0 mg/l BAP and 0.5 mg/l NAA gave the best result. Ninety percent of the explants induced multiple shoots within 10 to 14 days of inoculation with five maximum numbers of shoots per explant. The numbers of multiple shoots was low during initial subculture but increased after third subculture and were slightly decreased after fourth subculture. Rooting was spontaneous in almost all the treatments after 10 to 14 days of culture. Micropropagated plantlets were successfully acclimatized.

Item Type: Article
Uncontrolled Keywords: Boesenbergia rotunda Medicinal plant Micropropagation Zingiberaceae 1 naphthylacetic acid 6 n benzyladenine gelrite sucrose acclimatization article bud concentration (parameters) culture technique inoculation Murashige and Skoog medium nonhuman rhizome rooting shoot
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 07 May 2013 02:04
Last Modified: 07 May 2013 02:04
URI: http://eprints.um.edu.my/id/eprint/6007

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