Yusuf, N.A. and Annuar, M.S.M. and Khalid, N. (2012) Physical stress for overproduction of biomass and flavonoids in cell suspension cultures of boesenbergia rotunda. Acta Physiologiae Plantarum. pp. 1-7. ISSN 01375881, DOI https://doi.org/10.1007/s11738-012-1178-5.
Full text not available from this repository.Abstract
Flavonoid, a bioactive compound isolated from the rhizomes of Boesenbergia rotunda, exhibited antibiotic and anti-inflammatory properties and has also shown high inhibitory activity of dengue-2 virus protease. Several factors are responsible for the production of flavonoid in cell cultures. In the present study, the effects of initial inoculation volume, temperature and speed of agitation on cell growth, total and selected flavonoid in suspension cultures of B. rotunda were determined. High performance liquid chromatography analysis showed that a 2 inoculation volume induced a significantly high accumulation of biomass and of flavonoid in the cells. The cells cultured at 25 °C showed significantly high biomass and selected flavonoid accumulation while differences in medium agitation significantly affected the yield of selected flavonoid.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Abiotic stress Cell cultures Inoculation volume Secondary metabolites Zingiberaceae |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 07 May 2013 03:16 |
| Last Modified: | 07 May 2013 03:16 |
| URI: | http://eprints.um.edu.my/id/eprint/5992 |
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