Physical stress for overproduction of biomass and flavonoids in cell suspension cultures of boesenbergia rotunda

Yusuf, N.A. and Annuar, M.S.M. and Khalid, N. (2012) Physical stress for overproduction of biomass and flavonoids in cell suspension cultures of boesenbergia rotunda. Acta Physiologiae Plantarum. pp. 1-7. ISSN 01375881

Full text not available from this repository. (Request a copy)

Abstract

Flavonoid, a bioactive compound isolated from the rhizomes of Boesenbergia rotunda, exhibited antibiotic and anti-inflammatory properties and has also shown high inhibitory activity of dengue-2 virus protease. Several factors are responsible for the production of flavonoid in cell cultures. In the present study, the effects of initial inoculation volume, temperature and speed of agitation on cell growth, total and selected flavonoid in suspension cultures of B. rotunda were determined. High performance liquid chromatography analysis showed that a 2  inoculation volume induced a significantly high accumulation of biomass and of flavonoid in the cells. The cells cultured at 25 °C showed significantly high biomass and selected flavonoid accumulation while differences in medium agitation significantly affected the yield of selected flavonoid.

Item Type: Article
Uncontrolled Keywords: Abiotic stress Cell cultures Inoculation volume Secondary metabolites Zingiberaceae
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 07 May 2013 03:16
Last Modified: 07 May 2013 03:16
URI: http://eprints.um.edu.my/id/eprint/5992

Actions (login required)

View Item View Item