Enhancement of banana plant regeneration by incorporating a liquid-based embryo development Medium for embryogenic cell suspension

Wong, W.C. and Jalil, M. and Ong-Abdullah, M. and Othman, R.Y. and Khalid, N. (2006) Enhancement of banana plant regeneration by incorporating a liquid-based embryo development Medium for embryogenic cell suspension. Journal of Horticultural Science and Biotechnology, 81 (3). pp. 385-390. ISSN 14620316

Full text not available from this repository. (Request a copy)

Abstract

Recovering plants from embryogenic cell suspensions is a complex process starting with somatic embryo development which is synchronised on medium lacking plant growth regulators, followed by differentiation and regeneration of plants. An improved method for high frequency recovery of banana plants was investigated by incorporating a liquid-based, embryo-development medium. The highest regeneration rate (approx. 32,000 plants ml-1 settled cell volume) was obtained using this liquid protocol. This is one of the highest scores recorded among published data on plant recovery in Musa spp. A detailed, comparative histological study has been carried out on somatic embryos developed in semi-solid or liquid-based media. Most mature embryos developed from the liquid protocol enabled differentiation and regeneration within 4 months. Over 3,000 plants are currently under field observation to establish the rate of somaclonal variation.

Item Type: Article
Uncontrolled Keywords: Musa spp.,embryogenic,somatic embryos
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 29 Apr 2013 02:23
Last Modified: 29 Apr 2013 02:23
URI: http://eprints.um.edu.my/id/eprint/5945

Actions (login required)

View Item View Item