Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers

Zainuddin, Z. and Khalid, N. and Tan, C.S. (2002) Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers. Malaysian Journal of Science, 21 (1 & 2). pp. 25-30. ISSN 1394-3065

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Abstract

Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin (50 mg/l). Based on PCR analysis, transformation via A. tumefaciens was the best approach for introducing a novel chimeric gene into chilli tissues.

Item Type: Article
Uncontrolled Keywords: Capsicum annuum, Agrobacterium tumefaciens, particle bombardment, coat protein
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 17 Jan 2013 13:57
Last Modified: 17 Jan 2013 13:57
URI: http://eprints.um.edu.my/id/eprint/5899

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