Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers

Zainuddin, Z.; Khalid, N.; Tan, C.S. (2002) Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers. Malaysian Journal of Science, 21 (1 & 2). pp. 25-30. ISSN 1394-3065

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    Abstract

    Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin (50 mg/l). Based on PCR analysis, transformation via A. tumefaciens was the best approach for introducing a novel chimeric gene into chilli tissues.

    Item Type: Article
    Creators:
    1. Zainuddin, Z.
    2. Khalid, N.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    3. Tan, C.S.
    Journal or Publication Title: Malaysian Journal of Science
    Uncontrolled Keywords: Capsicum annuum, Agrobacterium tumefaciens, particle bombardment, coat protein
    Subjects: Q Science > QH Natural history > QH301 Biology
    Divisions: Faculty of Science > Institute of Biological Sciences
    Depositing User: miss munirah saadom
    Date Deposited: 17 Jan 2013 21:57
    Last Modified: 17 Jan 2013 21:57
    URI: http://eprints.um.edu.my/id/eprint/5899

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