Osteogenic genes related to the canonic WNT pathway are down-regulated in ameloblastoma

Sathi, G.A. and Tsujigiwa, H. and Ito, S. and Siar, C.H. and Katase, N. and Tamamura, R. and Harada, H. and Nagatsuka, H. (2012) Osteogenic genes related to the canonic WNT pathway are down-regulated in ameloblastoma. Oral Surgery Oral Medicine Oral Pathology Oral Radiology, 114 (6). pp. 771-777. ISSN 2212-4403


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Objective. The aim of this study was to determine the expression of essential osteogenic genes related to the canonic WNT pathway, i.e., WDR5, sFRP-2, and their downstream genes, in ameloblastoma and to clarify their biologic impact on this neoplasm. Study Design. Forty-six paraffin-embedded ameloblastoma samples and ameloblastic (AM-1) and preosteoblastic (KUSA/A1) cell lines were used. Immunohistochemistry, Western blot, reverse-transcription polymerase chain reaction, and alkaline phosphatase (ALP) activity assay were performed. Results. WDR5, essential for osteoblast differentiation and canonic WNT pathway activation, was negative in most ameloblastoma cases and weakly expressed in AM-1 cells. Conversely, sFRP-2s was overexpressed. RUNX2 and C-MYC, downstream inductions of canonic WNT pathway activation, demonstrated weak mRNA expressions in ameloblastoma, suggesting WNT pathway impairment and WDR5 functional inactivity. Recombinant WDR5 weakly induced ALP activity of KUSA/A1 cells cultured in AM-1 conditioned medium. Conclusions. These findings suggest that WNT-related bone-forming genes are down-regulated in ameloblastoma. Concurrent sFRP-2 overexpression suggests that both bone-forming and bone-inhibiting genes equally contributed to reduced bone formation in this neoplasm. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:771-777)

Item Type: Article
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Uncontrolled Keywords: wd-40 repeat protein beta-catenin bone-formation signaling pathway wd-repeat cell-line expression differentiation induce family
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 19 Apr 2013 00:04
Last Modified: 19 Apr 2013 00:04
URI: http://eprints.um.edu.my/id/eprint/5837

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