The biological seal of the implant-soft tissue interface evaluated in a tissue-engineered oral mucosal model

Chai, W.L. and Brook, I.M. and Palmquist, A. and van Noort, R. and Moharamzadeh, K. (2012) The biological seal of the implant-soft tissue interface evaluated in a tissue-engineered oral mucosal model. Journal of the Royal Society Interface, 9 (77). pp. 3528-3538. ISSN 1742-5689

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For dental implants, it is vital that an initial soft tissue seal is achieved as this helps to stabilize and preserve the peri-implant tissues during the restorative stages following placement. The study of the implant-soft tissue interface is usually undertaken in animal models. We have developed an in vitro three-dimensional tissue-engineered oral mucosal model (3D OMM), which lends itself to the study of the implant-soft tissue interface as it has been shown that cells from the three-dimensional OMM attach onto titanium (Ti) surfaces forming a biological seal (BS). This study compares the quality of the BS achieved using the three-dimensional OMM for four types of Ti surfaces: polished, machined, sandblasted and anodized (TiUnite). The BS was evaluated quantitatively by permeability and cell attachment tests. Tritiated water (HTO) was used as the tracing agent for the permeability test. At the end of the permeability test, the Ti discs were removed from the three-dimensional OMM and an Alamar Blue assay was used for the measurement of residual cells attached to the Ti discs. The penetration of the HTO through the BS for the four types of Ti surfaces was not significantly different, and there was no significant difference in the viability of residual cells that attached to the Ti surfaces. The BS of the tissue-engineered oral mucosa around the four types of Ti surface topographies was not significantly different.

Item Type: Article
Additional Information: 030LV Times Cited:0 Cited References Count:27
Uncontrolled Keywords: Permeability tissue-engineered oral mucosa implant-soft tissue interface biological seal three-dimensional in vitro model dental implant in-vitro keratinocyte cultures titanium implants peri-implant permeability water surfaces barrier cells
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of General Dental Practice And Oral & Maxillofacial Imaging
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 19 Apr 2013 01:08
Last Modified: 19 Apr 2013 01:08

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