Permethrin Resistance in Aedes aegypti (Linnaeus) Collected from Kuala Lumpur, Malaysia

Wan-Norafikah, O. and Nazni, W.A. and Lee, H.L. and Zainol-Ariffin, P. and Sofian-Azirun, M. (2010) Permethrin Resistance in Aedes aegypti (Linnaeus) Collected from Kuala Lumpur, Malaysia. Journal of Asia-Pacific Entomology, 13 (3). pp. 175-182. ISSN 12268615

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Abstract

Permethrin resistance status of a laboratory strain, a permethrin-selected strain and three field strains of Aedes aegypti collected in Kuala Lumpur, Malaysia were evaluated using three standard laboratory bioassays: WHO larval bioassay, WHO adult mosquito bioassay, and mixed function oxidase (MFO) enzyme microassay. The LC50 values of field strains from the WHO larval bioassay did not differ significantly. The highest LC50 value was from the Taman Melati field strain (0.39mg/L). The resistance ratio for the permethrin-selected strain and the field strains ranged from 1.86 fold to 5.57 fold. Pre-exposure to piperonyl butoxide (PBO) in the WHO adult bioassay and MFOs enzyme microassay reduced the LT50 values and reduced the mean optical density of elevated oxidase activity (0.28-0.42) at 630nm. The LC50 or LT50 values and the level of oxidases were significantly correlated (r=0.825; p<0.05). This study confirmed the presence of permethrin resistance in these mosquito populations. © 2010 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.

Item Type: Article
Uncontrolled Keywords: Aedes aegypti, Bioassay, Malaysia, Oxidases, Permethrin resistance.
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 14 Jan 2013 02:43
Last Modified: 16 Dec 2014 04:31
URI: http://eprints.um.edu.my/id/eprint/5813

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