Insecticide Susceptibility and Resistance Development in Malathion Selected Aedes albopictus (Skuse)

Selvi, S. and Edah, M.A. and Nazni, W.A. and Lee, H.L. and Tyagi, B.K. and Sofian-Azirun, Mohd and Azahari, A.H. (2010) Insecticide Susceptibility and Resistance Development in Malathion Selected Aedes albopictus (Skuse). Tropical Biomedicine, 27 (3). pp. 534-550. ISSN 0127-5720

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Abstract

Aedes albopictus was bioassayed to determine resistance development to malathion (OP). Two methods were applied, including WHO larval bioassay to determine the susceptibility to lethal concentration (LC), and adult bioassay to determine lethal time (LT). Larvae from colonies that had undergone selection pressure with malathion to yield 50 mortality were further subjected to selection for subsequent 10 generations. Selection of Ae. albopictus with malathion could relatively induce a consistent resistance ratio of 1.0 throughout 10 generations. It was noted that Ae. albopictus larvae showed less susceptibility to malathion compared to adults. The susceptibility test of adult mosquitoes to diagnostic dosage of 5.0 malathion-impregnated paper showed a variety of susceptibility to malathion when compared to the susceptible strain. Bioassay results indicated that the LT 50 values of malathion-selected Ae. albopictus ranged between 11.5 - 58.8 minutes for ten consecutive generations. Biochemical enzyme studies indicated that there was a significant difference (p < 0.05) in esterase level in malathion-selected mosquitoes compared to non-selected control. Electrophoretic patterns of non-specific esterases at different life stages in malathion-selected Ae. albopictus suggested that non-specific esterases do not play a role in resistance of malathion-selected Ae. albopictus.

Item Type: Article
Uncontrolled Keywords: esterase insecticide malathion Aedes animal article bioassay drug effect electrophoresis enzymology female genetic selection insecticide resistance survival time Animals Biological Assay Esterases Insecticides Selection, Genetic Survival Analysis Time Factors
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 14 Jan 2013 04:07
Last Modified: 13 Feb 2019 09:15
URI: http://eprints.um.edu.my/id/eprint/5808

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