Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae)

Sim, K S; Sri Nurestri, A M; Norhanom, A W (2010) Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae). African Journal of Pharmacy and Pharmacology, 4 (5). pp. 193-201.

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    Abstract

    The total phenolic content of the extracts of Pereskia bleo were assessed by the Folin-Ciocalteau’s method while the antioxidant activities were determined by three different assays, namely scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and ß-carotene method. In the present study, the ethyl acetate extract exhibited the highest total phenolic content (40.12 mg of GAEs/g of extract) and showed the strongest antioxidant activity in the ß-carotene bleaching assay significantly. Whilst, the hexane extract showed significantly the highest antioxidant activity when determined by scavenging effect on DPPH radicals (EC50 210 μg/ml) and the reducing power assay.

    Item Type: Article
    Creators:
    1. Sim, K S
    2. Sri Nurestri, A M
    3. Norhanom, A W
    Journal or Publication Title: African Journal of Pharmacy and Pharmacology
    Uncontrolled Keywords: Pereskia bleo; Cactaceae; antioxidant activity; phenolic content;
    Subjects: Q Science > Q Science (General)
    Divisions: Faculty of Science > Institute of Biological Sciences
    Depositing User: Zanaria Saupi Udin
    Date Deposited: 18 Mar 2011 13:20
    Last Modified: 18 Mar 2011 13:20
    URI: http://eprints.um.edu.my/id/eprint/568

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