Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae)

Sim, K.S. and Sri Nurestri, A.M. and Norhanom, A.W. (2010) Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae). African Journal of Pharmacy and Pharmacology, 4 (5). pp. 193-201.

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Abstract

The total phenolic content of the extracts of Pereskia bleo were assessed by the Folin-Ciocalteau’s method while the antioxidant activities were determined by three different assays, namely scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and ß-carotene method. In the present study, the ethyl acetate extract exhibited the highest total phenolic content (40.12 mg of GAEs/g of extract) and showed the strongest antioxidant activity in the ß-carotene bleaching assay significantly. Whilst, the hexane extract showed significantly the highest antioxidant activity when determined by scavenging effect on DPPH radicals (EC50 210 μg/ml) and the reducing power assay.

Item Type: Article
Uncontrolled Keywords: Pereskia bleo; Cactaceae; antioxidant activity; phenolic content;
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Zanaria Saupi Udin
Date Deposited: 18 Mar 2011 05:20
Last Modified: 30 Dec 2014 02:42
URI: http://eprints.um.edu.my/id/eprint/568

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