Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

Hasima, N.N. and Saidi, J. and Thong, Kwai Lin and Ansary, A. (2001) Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 147-150. ISSN 0128-7451

Full text not available from this repository. (Request a copy)
Official URL: http://www.msmbb.my/index.php/archive-issues

Abstract

Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200 V, ramped pulsed time of 1-25 s and a run time of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.

Item Type: Article
Uncontrolled Keywords: Pasteurella multocida; PFGE; restriction enzymes
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 04 Jan 2013 16:27
Last Modified: 08 Feb 2019 09:10
URI: http://eprints.um.edu.my/id/eprint/5663

Actions (login required)

View Item View Item