Technical Improvement in DNA Preparation for Pulsed-Field Gel Electrophoresis

Thong, K.L.; Lai, K.S.; Goh, Y.L. (2003) Technical Improvement in DNA Preparation for Pulsed-Field Gel Electrophoresis. Malaysian Journal of Science.

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We report a rapid pulsed-field electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results obtained using this modified, rapid protocol were highly reproducible.

Item Type: Article
  1. Thong, K.L.(Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Lai, K.S.
  3. Goh, Y.L.
Journal or Publication Title: Malaysian Journal of Science
Uncontrolled Keywords: pulsed-field gel electrophoresis, rapid method
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 05 Jan 2013 00:34
Last Modified: 14 Dec 2017 12:11

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