Technical Improvement in DNA Preparation for Pulsed-Field Gel Electrophoresis

Thong, Kwai Lin and Lai, K.S. and Goh, Y.L. (2003) Technical Improvement in DNA Preparation for Pulsed-Field Gel Electrophoresis. Malaysian Journal of Science. ISSN 1394-3065

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Abstract

We report a rapid pulsed-field electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results obtained using this modified, rapid protocol were highly reproducible.

Item Type: Article
Uncontrolled Keywords: pulsed-field gel electrophoresis, rapid method
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 04 Jan 2013 16:34
Last Modified: 27 Apr 2021 07:58
URI: http://eprints.um.edu.my/id/eprint/5660

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