Thong, Kwai Lin and Chan, Kok Gan and Devi, Shamala (2003) Responses of Salmonella enterica Serotype Typhi to Hydrogen Peroxide. Malaysian Journal of Science, 22 (2). pp. 41-48. ISSN 1394-3065,
Full text not available from this repository.Abstract
A strain of Salmonella enterica serotype Typhi (S. Typhi) used in this study can survive in high concentrations of hydrogen peroxide of up to 100mM. It could be adapted to grow in concentration of hydrogen peroxide of up to 250mM when the culture has been pre-treated with nonlethal concentration of hydrogen peroxide (60μ M). Adapted cells were found to be resistant to heat killing at 50°C. SDS-PAGE analysis showed that two outer membrane proteins of molecular weight of 45 and 68 kDa were synthesized when S. Typhi cells were challenged with 50mM hydrogen peroxide. Both of these OMPs were not present in untreated cell cultures. Immunoblotting experiments with sera from patients with a culture positive diagnosis of typhoid fever showed that these proteins were immunogenic.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Salmonella Typhi, Hydrogen peroxide, OMPs |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 04 Jan 2013 16:34 |
| Last Modified: | 23 Dec 2019 05:41 |
| URI: | http://eprints.um.edu.my/id/eprint/5659 |
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