Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia

Goh, Y.L. and Puthucheary, S.D. and Thong, Kwai Lin (2000) Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health. ISSN 0125-1562

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Abstract

A representative sample of 20 isolates of Salmonella weltevreden strains from stool cultures of patients admitted at the University Hospital, Kuala Lumpur, Malaysia were analyzed. All the strains were susceptible to ampicillin, ceftriaxone, ciprofloxacin, chloramphenicol, tetracycline, trimethoprim, gentamicin and co-trimoxazole. Ribosomal RNA gene restriction pattern analysis of PstI-digested DNA gave three ribotypes while pulsed-field gel electrophoresis (PFGE) analysis of XbaI-digested DNA gave ten distinct profiles. PFGE was more discriminative than ribotyping in distinguishing the strains. The majority of the strains analyzed were very closely related with similarity coefficient values ranging from 0.8 to 1.0. Both PFGE and ribotyping could distinguish one of the strains which was obtained from a patient following a bone marrow transplant for β-thalassemia major, indicating that this particular strain was unrelated to the rest of the strains from patients with acute gastroenteritis.

Item Type: Article
Uncontrolled Keywords: ribosomal RNA gene,pulsed-field gel electrophoresis,distinguishing Salmonella, weltevreden isolates
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 28 Dec 2012 05:08
Last Modified: 19 Oct 2018 08:26
URI: http://eprints.um.edu.my/id/eprint/5650

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