Role of Molecular Typing in an Outbreak of Salmonella paratyphi A

Chandel, D.S. and Nisar, N. and Thong, Kwai Lin and Pang, T. and Chaudhry, R. (2000) Role of Molecular Typing in an Outbreak of Salmonella paratyphi A. Tropical Gastroenterology, 21 (3). pp. 121-123. ISSN 0250-636X

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Abstract

During the post monsoon season of 1996 an outbreak of human Salmonellosis caused by Salmonella serovar-paratyphi A occurred in New Delhi and had continued for over 2 months. A total of 36 clinically diagnosed enteric-fever cases were reported during this outbreak. The isolates were compared following their characterisation by biotyping, antibiogram-analysis, plasmid-profiling and IS200 probing, to study the relatedness in order to delineate a common source. The study included representative strains from both outbreak (15) and sporadic (7) cases for comparative analysis. Biotyping, antibiogram, whole cell protein-analysis and plasmid-profiling could not discriminate sporadic cases from outbreak strains, suggesting that a single clone/type (PT-1) may be prevalent in our region. In contrast, molecular-typing using IS200-probing revealed 2 clonally related strains circulating during the outbreak, as compared to the unrelated sporadic strains which exhibited considerable genetic diversity. Molecular analysis by IS200-probing, helped to assign an index case which provided a history of later outbreaks, since paratyphi A was repeatedly cultured in later outbreaks also. The study also suggests that genetic rearrangements can occur during the emergence of outbreaks. It reaffirmed the usefulness of IS200-probing in epidemiological investigations of Salmonella enterica serovars.

Item Type: Article
Uncontrolled Keywords: Bacterial Typing Techniques Disease Outbreaks Humans India —epidemiology Microbial Sensitivity Tests Paratyphoid Fever —epidemiology Salmonella paratyphi A —classification
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 05 Jan 2013 11:47
Last Modified: 12 Oct 2018 06:30
URI: http://eprints.um.edu.my/id/eprint/5623

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