Ho, W.S. and Balan, G. and Puthucheary, S. and Kong, B.H. and Lim, K.T. and Tan, L.K. and Koh, X.P. and Yeo, C.C. and Thong, Kwai Lin (2012) Prevalence and Characterization of Multidrug-Resistant and Extended-Spectrum Beta-Lactamase-Producing Escherichia coli from Pediatric Wards of a Malaysian Hospital. Microbial Drug Resistance, 18 (4). pp. 408-416. ISSN 1076-6294, DOI https://doi.org/10.1089/mdr.2011.0222.
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Abstract
The emergence of Escherichia coli resistant to extended-spectrum cephalosporins (ESCs) is of concern as ESC is often used to treat infections by Gram-negative bacteria. One-hundred and ten E. coli strains isolated in 2009-2010 from children warded in a Malaysian tertiary hospital were analyzed for their antibiograms, carriage of extended-spectrum beta-lactamase (ESBL) and AmpC genes, possible inclusion of the beta-lactamase genes on an integron platform, and their genetic relatedness. All E. coli strains were sensitive to carbapenems. About 46 of strains were multidrug resistant (MDR; i.e., resistant to >= 3 antibiotic classes) and almost half (45) were nonsusceptible to ESCs. Among the MDR strains, high resistance rates were observed for ampicillin (98), tetracycline (75), and trimethoprim/sulfamethoxazole (73). Out of 110 strains, bla(TEM-1) (49.1), bla(CTX-M) (11.8), and bla(CMY-2) (6.4) were detected. Twenty-one strains were ESBL producers. CTX-M-15 was the predominant CTX-M variant found and this is the first report of a CTX-M-27-producing E. coli strain from Malaysia. Majority (3.1) of the strains harbored class 1 integron-encoded integrases with a predominance of aadA and dfr genes within the integron variable region. No gene cassette encoding ESBL genes was found and integrons were not significantly associated with ESBL or non-ESBL producers. Possible clonal expansion was observed for few CTX-M-15-positive strains but the O25-ST131 E. coli clone known to harbor CTX-M-15 was not detected while CMY-2-positive strains were genetically diverse.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 05 Jan 2013 14:45 |
| Last Modified: | 15 Jul 2019 04:00 |
| URI: | http://eprints.um.edu.my/id/eprint/5605 |
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