Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis

Thong, Kwai Lin and Goh, Yee Ling and Phung, Dac Cam (2002) Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis. Asia Pacific Journal of Molecular Biology and Biotechnology, 10 (2). pp. 103-108. ISSN 0128-7451

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Abstract

Pulsed-field gel electrophoresis (PFGE) was applied to analyse sporadic and outbreak cases of cholera caused by V. cholerae O1 biotype El Tor from Vietnam (n=16) and V. cholerae O139 from Malaysia (n=12) and from five vaccine strains. NotI-Digestion of chromosomal DNA from these isolates, followed by PFGE produced eleven different profiles among the 16 V. cholerae O1 isolates (Dice coefficient: 0.67-0.97). The V. cholerae O1 Ogawa and Inaba strains were clearly differentiated into distinct clusters. NotI-PFGE analysis among V. cholerae O139 isolates showed a predominant PFGE profile (N12) indicating that the outbreak was probably derived a single clone. Among the five vaccine strains, the PFGE profiles of V. cholerae Inaba Cairo 48 and classical Inaba Cairo 48 were similar to some of the field isolates of 01 Inaba strains in Vietnam. The PFGE analysis showed that the V. cholerae O139 isolates from Malaysia were indistinguishable as these strains were probably derived from a cholera outbreak and the V. cholerae O1 isolates from Vietnam exhibited limited genetic diversity.

Item Type: Article
Uncontrolled Keywords: Pulsed-field gel electrophoresis; PFGE; Vibrio cholerae O1 and O139
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 16 Apr 2013 02:29
Last Modified: 11 Feb 2019 07:47
URI: http://eprints.um.edu.my/id/eprint/5586

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