Evaluation of the H1d flagellin gene targeting pcr for detection of salmonella enterica serovar typhi

Thong, K.L.; Pathmanathan, S.G.; Puthucheary, S.D. (2005) Evaluation of the H1d flagellin gene targeting pcr for detection of salmonella enterica serovar typhi. Malaysian Journal of Science, 24 (1). pp. 1-7. ISSN 1394-3065

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Abstract

A nested-PCR assay targeting the H1-d flagellin gene was evaluated on 46 Salmonella strains representing 27 different serovars and 13 non-Salmonella strains from eight different bacterial genera. The PCR was highly sensitive as it could detect down to 2 cfu/ml after the nested PCR. The first and second round of PCR amplifications produced the expected amplicons in all the S. Typhi as well as S. Muenchen and S. Stanley strains. The second round of PCR also produced an amplicon of the expected size with the Citrobacter freundii strain. These findings indicate that the PCR is not completely specific for S. Typhi. The nested-PCR system also posed problems of carryover contaminations. Although highly sensitive, the problem of specificity and carryover contamination questions the reliability of this nested-PCR assay. Therefore there is a need for the development of new PCR assays targeting more specific regions of the S. Typhi genome.

Item Type: Article
Creators:
  1. Thong, K.L.(Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Pathmanathan, S.G.
  3. Puthucheary, S.D.
Journal or Publication Title: Malaysian Journal of Science
Uncontrolled Keywords: Flagellin gene, S. Typhi, detection
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 11 Apr 2013 09:43
Last Modified: 14 Dec 2017 12:21
URI: http://eprints.um.edu.my/id/eprint/5519

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