Lim, K.T. and Hanifah, Y.A. and Yusof, M.Y.M. and Thong, Kwai Lin (2012) ErmA, ermC, tetM and tetK are essential for erythromycin and tetracycline resistance among methicillin-resistant staphylococcus aureus strains isolated from a tertiary Hospital in Malaysia. Indian Journal of Medical Microbiology, 30 (2). pp. 203-207. ISSN 0255-0857, DOI https://doi.org/10.4103/0255-0857.96693.
Full text not available from this repository.Abstract
The objective of this study was to determine the expression and transferability of tetracycline and erythromycin resistance among 188 MRSA strains from a Malaysian tertiary hospital. The minimum inhibitory concentrations (MICs) for oxacillin, erythromycin, tetracycline and ciprofloxacin ranged from 4 to 512 mu g/ml, 0.25 to 256 mu g/ml, 0.5 to 256 mu g/ml and 0.5 to 512 mu g/ml, respectively. Tetracycline-resistant strains showed co-resistance towards ciprofloxacin and erythromycin. There was a significant increase (P<0.05) of high-level tetracycline (>= MIC 256 mu g/ml) and erythromycin (>= MIC 128 mu g/ml) resistant strains in between the years 2003 and 2008. All erythromycin-resistant strains harboured ermA or ermC gene and all tetracycline-resistant strains harboured tetM or tetK gene. The blaZ was detected in all MRSA strains, whereas ermA, tetM, ermC, tetK and msrA genes were detected in 157 (84), 92 (49), 40 (21), 39 (21) and 4 (2) MRSA strains, respectively. The blaZ, tetM, ermC and tetK genes were plasmid-encoded, with ermC gene being easily transmissible. Tn5801-like transposon was present in 78 tetM-positive strains. ermA and tetM genes were the most prevalent erythromycin and tetracycline resistance determinants, respectively, in MRSA strains. The association of resistance genes with mobile genetic elements possibly enhances the spread of resistant traits in MRSA.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Ciprofloxacin, erythromycin, Methicillin-resistant Staphylococcus aureus, tetracycline, transformation |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 12 Apr 2013 01:26 |
| Last Modified: | 15 Oct 2018 05:14 |
| URI: | http://eprints.um.edu.my/id/eprint/5518 |
Actions (login required)
 |
View Item |
