Paramanantham, Yogaambikai and Said, Nur Akmarina B. M. and Mun, Kein Seong (2023) The correlation of EMT and p53 immunohistochemical markers with cisplatin resistance in muscle invasive bladder cancer patients: A single-centred study. Malaysian Journal of Pathology, 45 (1). pp. 19-29. ISSN 0126-8635,
Full text not available from this repository.Abstract
Introduction: Although epithelial-mesenchymal transition (EMT) and p53 have been established to play a pivotal role in the aggressiveness of muscle-invasive bladder cancer (MIBC), its pathological correlation to cisplatin treatment in the Malaysian patient cohort is lacking. This study aimed to evaluate the association of EMT markers, e-cadherin, vimentin and actin, as well as tumour suppressor gene, p53, in cisplatin-receiving MIBC patients. Materials and Methods: Formalin-fixed paraffinembedded (FFPE) blocks of muscle-invasive bladder cancer patients receiving cisplatin-based chemotherapy between January 2010 to December 2020 were traced. Immunohistochemistry staining was performed on traced blocks using antibodies to e-cadherin, vimentin and actin, and p53. Results: p53 and e-cadherin were stained positive in most cases (p=0.515 and 0.242 respectively), although e-cadherin showed stronger positive expression in pre-cisplatin receiving MIBC cases. All the cases stained negative for actin and vimentin except for faint staining observed in one pre-cisplatin case. Conclusion: Although this study does not show a significant correlation between EMT markers and p53 with cisplatin-responsiveness in MIBC patients, the results serve as preliminary findings on the heterogeneous outcomes of molecular staining in the Malaysian MIBC patient cohort.
| Item Type: | Article |
|---|---|
| Funders: | Malaysian Ministry of Higher Education: Impact -Oriented Interdisciplinary Research Grant Programme IIRG (IIRG022A-2019), Malaysian Ministry of Higher Education: Fundamental Research Grant Scheme FRGS (10770) ; (FRGS/1/2016/SKK08/UM/02/22) |
| Uncontrolled Keywords: | Muscle -invasive bladder cancer; epithelial-mesenchymal transition; cisplatin resistance |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine > Pathology Department Faculty of Pharmacy > Department of Pharmaceutical Life Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 22 Jul 2025 00:55 |
| Last Modified: | 22 Jul 2025 00:55 |
| URI: | http://eprints.um.edu.my/id/eprint/50836 |
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