Kaur, Christina Parvinder and Yong, Chia Chai and Rajamanikam, Arutchelvan and Samudi, Chandramathi and Kumar, Suresh and Bhassu, Subha and Mohamad, Saharuddin and Venugopal, Kavilasha and Kareem, Sameem Abdul (2023) The role and influence of bacteria on the growth of Blastocystis sp. subtype 3. Parasitology Research, 122 (7). pp. 1463-1474. ISSN 0932-0113, DOI https://doi.org/10.1007/s00436-023-07842-2.
Full text not available from this repository.Abstract
Blastocystis sp. is an enteric protistan parasite that affects individuals worldwide with gastrointestinal symptoms such as abdominal discomfort, diarrhea, and flatulence. However, its pathogenicity is controversial due to its presence among asymptomatic individuals. Blastocystis sp. subtype 3 (ST3) is the most prevalent subtype among humans that have been associated with irritable bowel syndrome (IBS), Crohn's disease, ulcerative colitis, and colorectal cancer. Axenization of the parasite has been shown to impede its growth thus revealing the importance of accompanying bacteria in ensuring Blastocystis sp. survival. This study aims to identify the influence of accompanying bacteria on the growth of Blastocystis sp. ST3. Blastocystis sp. cultures were treated with Meropenem, Vancomycin, and Amoxicillin-Clavulanic acid (Augmentin). Bacteria-containing supernatant of antibiotic-treated and control cultures were isolated and identified through 16 s rRNA sequencing. Morphological changes of antibiotic-treated Blastocystis sp. ST3 were also observed. The cultures treated with meropenem and augmentin exhibited opposing effects with reduced growth of isolates from symptomatic patients and a significant increase in asymptomatic isolates. Whereas, vancomycin-treated cultures had no difference in the growth of Blastocystis sp. ST3 isolates from symptomatic and asymptomatic patients. Isolates from symptomatic and asymtomatic patients had 6 and 2 distinct bacterial species identified with Proteus mirabilis as the common bacteria among both types of isolates. Morphologically, Blastocystis sp. ST3 cultures exposed to meropenem and augmentin demonstrated an increase in pre-cystic forms. These findings demonstrate the effects of accompanying bacteria on the growth of Blastocystis sp. ST3 that could translate into clinical manifestations observed among Blastocystis sp.-infected patients.
| Item Type: | Article |
|---|---|
| Funders: | Trans-disciplinary Research Grant Scheme (TRGS) of the Ministry of Higher Education (MOHE), Malaysia (TRGS/1/2018/UM/01/7/3) |
| Uncontrolled Keywords: | Blastocystis sp; ST3; Accompanying bacteria; Morphology; Pre-cystic; Antibiotic treatment |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Divisions: | Faculty of Computer Science & Information Technology Faculty of Medicine > Biomedical Science Department Faculty of Medicine > Medical Microbiology Department Faculty of Medicine > Parasitology Deparment Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Jul 2025 03:38 |
| Last Modified: | 28 Jul 2025 03:38 |
| URI: | http://eprints.um.edu.my/id/eprint/50791 |
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