Kaur, Christina Parvinder and Iyadorai, Thevambiga and Sears, Cynthia and Roslani, April Camilla and Vadivelu, Jamuna and Samudi, Chandramathi (2023) Presence of Polyketide Synthase (PKS) Gene and Counterpart Virulence Determinants in Klebsiella pneumoniae Strains Enhances Colorectal Cancer Progression In-Vitro. Microorganisms, 11 (2). ISSN 2076-2607, DOI https://doi.org/10.3390/microorganisms11020443.
Full text not available from this repository.Abstract
Klebsiella pneumoniae (K. pneumoniae) colonizes the human gut and is a causative factor of pyogenic liver abscess (PLA). Retrospective studies conducted on K. pneumoniae PLA patients revealed subsequent CRC development in later years of their life with increasing prevalence of these strains harbouring polyketide synthase (PKS) genes. To our knowledge there are no known studies directly implicating K. pneumoniae with CRC to date. Our aims are to characterize K. pneumoniae isolates from CRC patients and investigate its effects on cell proliferation in vitro. K. pneumoniae isolates were characterized by screening virulence genes including polyketide synthase (PKS), biofilm assay, antibiotic susceptibility, and string test to determine hypervirulent (hvKp) strains. Solubilised antigens of selected K. pneumoniae isolates were co-cultured with primary colon cell lines and CRC cell lines (Stage I-IV) for 48 h. The enhancement of proliferation was measured through MTT and ECIS assay. Twenty-five percent of K. pneumoniae isolates were PKS-positive out of which 50% were hvKp strains. The majority of the isolates were from the more virulent serotype of K1 (30%) and K2 (50%). PKS-positive K. pneumoniae isolates did not possess genes to confer carbapenem resistance but instead were more highly associated with siderophore genes (aerobactin, enterobactin, and yersiniabactin) and allantoin metabolism genes (allS, allS2). Cell proliferation in primary colon, SW1116 (Stage I), and SW480 (Stage II) CRC cell lines were enhanced when co-cultured with PKS-positive K. pneumoniae antigens. ECIS revealed enhanced cell proliferation upon recurrent antigen exposure. This demonstrates the possible role that PKS-positive K. pneumoniae has in exacerbating CRC progression.
| Item Type: | Article |
|---|---|
| Funders: | Trans-disciplinary Research Grant Scheme (TRGS) (Grant No: TR001C-2018A), Fundamental Research Grant Scheme (FRGS) (Grant No: FP040-2014B) of the Malaysian Ministry of Higher Education (MOHE) |
| Uncontrolled Keywords: | K; pneumoniae; colorectal cancer; polyketide synthase (PKS); colibactin; hypervirulent K; pneumoniae (hvKp); pyogenic liver abscess (PLA); siderophores; biofilm; string test; antibiotic resistance; electrical cell-substrate impedance sensing (ECIS) |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Medical Microbiology Department Faculty of Medicine > Surgery Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Sep 2025 03:51 |
| Last Modified: | 18 Sep 2025 03:51 |
| URI: | http://eprints.um.edu.my/id/eprint/50446 |
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