Tan, J. H. and Cheong, F. W. and Lau, Y. L. and Fong, M. Y. (2023) Plasmodium knowlesi circumsporozoite protein: genetic characterisation and predicted antigenicity of the central repeat region. Tropical Biomedicine, 40 (1). pp. 37-44. ISSN 0127-5720, DOI https://doi.org/10.47665/tb.40.1.004.
Full text not available from this repository.Abstract
Circumsporozoite protein (CSP) central repeat region is one of the main target regions of the RTS,S/AS01 vaccine for falciparum infection as it consists of immunodominant B cell epitopes. However, there is a lack of study for P. knowlesi CSP central repeat region. This study aims to characterise the CSP repeat motifs of P. knowlesi isolates in Peninsular Malaysia. CSP repeat motifs of 64 P. knowlesi isolates were identified using Rapid Automatic Detection and Alignment of Repeats (RADAR). Antigenicity of the repeat motifs and linear B cell epitopes were predicted using VaxiJen 2.0, BepiPred-2.0 and BCPred, respectively. A total of 35 dominant repeat motifs were identified. The repeat motif ``AGQPQAQGDGANAGQPQAQGDGAN'' has the highest repeat frequency (n=15) and antigenicity index of 1.7986. All the repeat regions were predicted as B cell epitopes. In silico approaches revealed that all repeat motifs were antigenic and consisted of B cell epitopes which could be designed as knowlesi malaria vaccine.
| Item Type: | Article |
|---|---|
| Funders: | Long Term Research Grant Scheme from the Ministry of Higher Education, Malaysia (LRGS/1/2018/UM/01/1/1) |
| Uncontrolled Keywords: | Circumsporozoite protein; central repeat; P; knowlesi; genetic characterisation; B-cell epitopes |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Sep 2025 07:38 |
| Last Modified: | 18 Sep 2025 07:38 |
| URI: | http://eprints.um.edu.my/id/eprint/50397 |
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