Wong, Jo-Ern and Zainal, Nurhafiza and AbuBakar, Sazaly and Tan, Kim-Kee (2023) Monitoring changes in human umbilical vein endothelial cells upon viral infection using impedance-based real-time cell analysis. Jove-Journal of Visualized Experiments (195). ISSN 1940-087X, DOI https://doi.org/10.3791/64887.
Full text not available from this repository.Abstract
Endothelial cells line the inner surface of all blood and lymphatic vessels, creating a semi-permeable barrier regulating fluid and solute exchange between blood or lymph and their surrounding tissues. The ability of a virus to cross the endothelial barrier is an important mechanism that facilitates virus dissemination in the human body. Many viruses are reported to alter endothelial permeability and/or cause endothelial cell barrier disruption during infection, which is able to cause vascular leakage. The current study describes a real-time cell analysis (RTCA) protocol, using a commercial real-time cell analyzer to monitor endothelial integrity and permeability changes during Zika virus (ZIKV) infection of the human umbilical vein endothelial cells (HUVECs). The impedance signals recorded before and after ZIKV infection were translated to cell index (CI) values and analyzed. The RTCA protocol allows the detection of transient effects in the form of cell morphological changes during a viral infection. This assay could also be useful for studying changes in the vascular integrity of HUVECs in other experimental setups.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education Malaysia under the Higher Institution Centre of Excellence (HICoE) program [Grant No: MO002-2019], Long -Term Research Grant Scheme (LRGS MRUN Phase 1) [Grant No: LRGS MRUN/F1/01/2018] |
| Uncontrolled Keywords: | Barrier function; Virus; Integrity; Migration system |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 06 Nov 2025 01:59 |
| Last Modified: | 06 Nov 2025 01:59 |
| URI: | http://eprints.um.edu.my/id/eprint/49814 |
Actions (login required)
 |
View Item |
