Lai, Meng Yee and Hamid, Mohd Abdul and Jelip, Jenarun and Mudin, Rose Nani and Lau, Yee Ling (2023) Lateral flow recombinase polymerase amplification assays for the detection of human plasmodium species. American Journal of Tropical Medicine and Hygiene, 108 (5). pp. 882-886. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.22-0657.
Full text not available from this repository.Abstract
This study highlights the development of two lateral flow recombinase polymerase amplification assays for the diagnosis of human malaria. The lateral flow cassettes contained test lines that captured biotin-, 6-carboxyfluorescein, digoxigenin-, cyanine 5-, and dinitrophenyl-labeled amplicons. The overall process can be completed in 30 minutes. Recombinase polymerase amplification coupled with lateral flow had a detection limit of 1 copy/mL for Plasmodium knowlesi, Plasmodium vivax, and Plasmodium falciparum. No cross-reactivity was observed among nonhuman malaria parasites such as Plasmodium coatneyi, Plasmodium cynomolgi, Plasmodium brasilanium, Plasmodium inui, Plasmodium fragile, Toxoplasma gondii, Sarcocystis spp., Brugia spp., and 20 healthy donors. It is rapid, highly sensitive, robust, and easy to use. The result can be read without the need for special equipment and thus has the potential to serve as an effective alternative to polymerase chain reaction methods for the diagnosis of malaria.
| Item Type: | Article |
|---|---|
| Funders: | Long Term Research Grant Scheme (LRGS) [Grant No: LRGS/1/2018/UM/01/1/4 (LR002D-2018)], Ministry of Education, Malaysia |
| Uncontrolled Keywords: | Malaria parasites; Rapid detection; Falciparum; Identification |
| Subjects: | Q Science > QR Microbiology R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 06 Nov 2025 02:09 |
| Last Modified: | 06 Nov 2025 02:09 |
| URI: | http://eprints.um.edu.my/id/eprint/49810 |
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