Comparison of dynamic responses of cellular metabolites in escherichia coli to pulse addition of substrates

Merican, A.F. and Hoque, M.A. and Fard, A.T. and Rahman, M. and Alattas, O. and Akazawa, K. (2011) Comparison of dynamic responses of cellular metabolites in escherichia coli to pulse addition of substrates. Biologia, 66 (6). pp. 954-966. ISSN 0006-3088

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Official URL: http://link.springer.com/article/10.2478%2Fs11756-...

Abstract

We conducted an integrated study of cell growth parameters, product formation, and the dynamics of intracellular metabolite concentrations using Escherichia coli with genes knocked out in the glycolytic and oxidative pentose phosphate pathway (PPP) for glucose catabolism. We investigated the same characteristics in the wild-type strain, using acetate or pyruvate as the sole carbon source. Dramatic effects on growth parameters and extracellular and intracellular metabolite concentrations were observed after blocking either glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (disruption of pgi gene) or pentose phosphate breakdown of glucose by inactivation of glucose-6-phosphate dehydrogenase (disruption of zwf gene). Reducing power (NADPH) was mainly produced through PPP when the pgi gene was knocked out, while NADPH was produced through the tricarboxylic acid (TCA) cycle by isocitrate dehydrogenase or NADP-linked malic enzyme when the zwf gene was knocked out. As expected, when the pgi gene was knocked out, intracellular concentrations of PPP metabolites were high and glycolytic and concentrations of TCA cycle pathway metabolites were low. In the zwf gene knockout, concentrations of PPP metabolites were low and concentrations of intracellular glycolytic and TCA cycle metabolites were high.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Pulse addition,dynamic response,metabolic engineering,gene knockout,pgi mutant,zwf mutant,metabolite concentration,zwf gene-knockout,in-vivo analysis,saccharomyces-cerevisiae,c-13-labeling experiments,phosphoglucose isomerase,continuous,cultures,chemostat cultures,bacillus-subtilis,enzyme-activities,flux,analysis
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 28 Feb 2013 00:51
Last Modified: 14 Jul 2017 06:59
URI: http://eprints.um.edu.my/id/eprint/4937

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