A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes.

Phua, Alice Choon Yen; Abdullah, Ramli Bin; Mohamed, Zulqarnain (2003) A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. The Journal of reproduction and development, 49 (4). pp. 307-11. ISSN 0916-8818

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Abstract

Sex determination of livestock is performed to achieve the objectives of livestock breeding programmes. Techniques for sex determination have evolved from karyotyping to detecting Y-specific antigens and recently to the polymerase chain reaction (PCR), which appears to be the most sensitive, accurate, rapid and reliable method to date. In this study, a PCR-based sex determination method for potential application in goat breeding programmes was developed. Primers were designed to amplify a portion of the X amelogenin gene (Aml-X) on the X chromosome to give a 300 bp product and Sry gene on the Y chromosome to give a 116 bp product. PCR optimization was performed using DNA template extracted from a whole blood sample of Jermasia goats (German Fawn x Katjang) of both sexes. It was possible to identify the sex chromosomes by amplifying both male- and female-specific genes simultaneously in a duplex reaction with males yielding two bands and females yielding one band. The Aml-X primer set, which served as an internal control primer, did not interfere with amplification of the Y-specific sequence even when a low amount of DNA (1 ng) was used. The duplex reaction subjected to a blind test showed 100% (14/14) concordance, proving its accuracy and reliability. The primer sets used were found to be highly specific and were suitable for gender selection of goats.

Item Type: Article
Creators:
  1. Phua, Alice Choon Yen
  2. Abdullah, Ramli Bin
  3. Mohamed, Zulqarnain
Journal or Publication Title: The Journal of reproduction and development
Uncontrolled Keywords: Amelogenin; Animals;
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Science
Depositing User: Mr. Faizal Hamzah
Date Deposited: 15 Mar 2011 11:28
Last Modified: 15 Mar 2011 11:28
URI: http://eprints.um.edu.my/id/eprint/488

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