Loo, Yan Shan and Zahid, N. Idayu and Madheswaran, Thiagarajan and Ikeno, Shinya and Nurdin, Armania and Azmi, Intan Diana Mat (2023) Coencapsulation of gemcitabine and thymoquinone in citrem-phosphatidylcholine hexosome nanocarriers improves in vitro cellular uptake in breast cancer cells. Molecular Pharmaceutics, 20 (9). pp. 4611-4628. ISSN 1543-8384, DOI https://doi.org/10.1021/acs.molpharmaceut.3c00333.
Full text not available from this repository.Abstract
Lyotropicliquid crystalline nanoassemblies (LLCNs) areinternallyself-assembled (ISA)-somes formed by amphiphilic molecules in a mixturecomprising a lipid, stabilizer, and/or surfactant and aqueous media/dispersant.LLCNs are unique nanoassemblies with versatile applications in a widerange of biomedical functions. However, they comprise a nanosystemthat is yet to be fully explored for targeted systemic treatment ofbreast cancer. In this study, LLCNs proposed for gemcitabine and thymoquinone(Gem-TQ) co-delivery were prepared from soy phosphatidylcholine (SPC),phytantriol (PHYT), or glycerol monostearate (MYVR) in optimized ratioscontaining a component of citric and fatty acid ester-based emulsifier(Grinsted citrem) or a triblock copolymer, Pluronic F127 (F127). Hydrodynamicparticle sizes determined were below 400 nm (ranged between 96 and365 nm), and the series of nanoformulations displayed negative surfacecharge. Nonlamellar phases identified by small-angle X-ray scattering(SAXS) profiles comprise the hexagonal, cubic, and micellar phases.In addition, high entrapment efficiency that accounted for 98.3 & PLUSMN;0.1% of Gem and 99.5 & PLUSMN; 0.1% of TQ encapsulated was demonstratedby the coloaded nanocarrier system, SPC/citrem/Gem-TQ hexosomes. Lowcytotoxicity of SPC-citrem hexosomes was demonstrated in MCF10A cellsconsistent with hemo- and biocompatibility observed in zebrafish (Danio rerio) embryos for up to 96 h postfertilization (hpf).SPC/citrem/Gem-TQ hexosomes demonstrated IC50 of 24.7 & PLUSMN;4.2 & mu;M in MCF7 breast cancer cells following a 24 h treatmentperiod with the moderately synergistic interaction between Gem andTQ retained (CI = 0.84). Taken together, biocompatible SPC/citrem/Gem-TQhexosomes can be further developed as a multifunctional therapeuticnanodelivery approach, plausible for targeting breast cancer cellsby incorporation of targeting ligands.
| Item Type: | Article |
|---|---|
| Funders: | Malaysia Toray Science Foundation (MTSF) Science and Technology Research Grant, Universiti Putra Malaysia [Grant No: 9734400] |
| Uncontrolled Keywords: | Lyotropic liquidcrystalline nanoparticles; Soy phosphatidylcholine; Gemcitabine; Thymoquinone; Combination therapy |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 01 Nov 2025 12:27 |
| Last Modified: | 01 Nov 2025 12:27 |
| URI: | http://eprints.um.edu.my/id/eprint/48661 |
Actions (login required)
 |
View Item |
