Mohamad Ikbal, Nurul Huda and Bhassu, Subha and Teh, Lay Kek and Salleh, Mohd Zaki and Chan, Chew Chin and Simarani, Khanom and Omar, Hasmahzaiti (2023) Differential expression of six cytokines in Rattus rattus exposed to leptospirosis: A comprehensive transcriptomic analysis. Cytokine, 172. ISSN 1043-4666, DOI https://doi.org/10.1016/j.cyto.2023.156402.
Full text not available from this repository.Abstract
Background: Rattus rattus are the main carriers of various zoonotic diseases including leptospirosis. Regrettably, information underlying the cytokine response of wild R. rattus upon leptospirosis infection is lacking. This study aims to understand the immune response presented by specifically the kidney and liver of R. rattus during leptospirosis infection.Methodology: High-throughput RNA-Sequencing technology was employed to discover the transcriptome alterations in the kidney and liver of R. rattus during natural infection. Both kidney and liver tissues from the healthy and infected rats were sequenced using the BGISEQ-500 sequencing platform. The GO and KEGG databases were utilized to functionally annotate the differentially expressed transcripts of the selected cytokines; TNF-alpha, IL-1 beta, IL-6, IL-10, MIP-1 alpha, and IFN-gamma.Results: A higher number of upregulated genes were signified in the kidney as compared to the liver during infection. Among the six selected cytokines, Interleukin-6 was found to be expressed during the early stage in the liver of R. rattus, while all the other six genes were upregulated during the late stage of leptospirosis in the kidney of R. rattus. The GO of the annotated genes was classified under inflammatory response and cellular response to lipopolysaccharide, while the KEGG pathway indicated cytokine-cytokine receptor interaction and the Toll-like receptor (TLR) signalling pathway. The upshots of this study correlated the different phases of cytokine response in different organs of R. rattus during leptospirosis infection.Conclusion: Overall, these studies formulate a conceptual framework based on host and pathogen relationships of leptospirosis transmission patterns and the discovery of biomarkers in tracking the early stages of leptospires colonization.
| Item Type: | Article |
|---|---|
| Funders: | Postgraduate Research Fund, PPP [Grant No: PG061-2016A], CEBAR Fund RU2017 University of Malaya, Kuala Lumpur, Malaysia [Grant No: RU006-2017] |
| Uncontrolled Keywords: | Interferon-gamma; Interleukins; Macrophage Inflammatory Protein-1 alpha; Tumor Necrosis Factor; Wild rats |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology > QR180 Immunology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Nov 2025 02:32 |
| Last Modified: | 03 Nov 2025 02:32 |
| URI: | http://eprints.um.edu.my/id/eprint/48596 |
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