Karajacob, Alexandria Sonia and Azizan, Nuramirah and Al-Maleki, Anis Rageh Mohammad and Goh, Joanne Pei En and Loke, Mun Fai and Khor, Hui Min and Ho, Gwo Fuang and Ponnampalavanar, Sasheela and Tay, Sun Tee (2023) Candida species and oral mycobiota of patients clinically diagnosed with oral thrush. PLoS ONE, 18 (4). ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0284043.
Full text not available from this repository.Abstract
Overgrowth of Candida yeasts in the oral cavity may result in the development of oral thrush in immunocompromised individuals. This study analyzed the diversity and richness of the oral mycobiota of patients clinically diagnosed with oral thrush (OT), follow-up of oral thrush patients after antifungal therapy (AT), and healthy controls (HC). Oral rinse and oral swab samples were collected from 38 OT patients, 21 AT patients, and 41 healthy individuals (HC). Pellet from the oral rinse and oral swab were used for the isolation of oral Candida yeasts on Brilliance Candida Agar followed by molecular speciation. ITS1 amplicon sequencing using Illumina MiSeq was performed on DNA extracted from the oral rinse pellet of 16 OT, 7 AT, and 7 HC oral rinse samples. Trimmed sequence data were taxonomically grouped and analyzed using the CLC Microbial Genomics Module workflow. Candida yeasts were isolated at significantly higher rates from oral rinse and swab samples of OT (68.4%, p < 0.001) and AT (61.9%, p = 0.012) patients, as compared to HC (26.8%). Predominance of Candida albicans specifically, was noted in OT (60.5%, p < 0.001) and AT (42.9%, p = 0.006) vs. HC (9.8%), while non-albicans Candida species was dominant in HC. Analysis of oral mycobiota from OT patients showed the presence of 8 phyla, 222 genera, and 309 fungal species. Low alpha diversity (Shannon index, p = 0.006; Chao-1 biased corrected index, p = 0.01), varied beta diversity (Bray-Curtis, p = 0.01986; Jaccard, p = 0.02766; Weighted UniFrac, p = 0.00528), and increased relative abundance of C. albicans (p = 3.18E-02) was significantly associated with the oral mycobiota of OT vs. HC. This study supported that C. albicans is the main etiological agent in oral thrush and highlights the association of fungal biodiversity with the pathophysiology of oral thrush.
| Item Type: | Article |
|---|---|
| Funders: | University Malaya Medical Centre, University Malaya |
| Uncontrolled Keywords: | Agar; Antifungal agents; Candida; Candida albicans; Candidiasis, Oral; Humans |
| Subjects: | R Medicine R Medicine > RK Dentistry |
| Divisions: | Faculty of Dentistry > Department of Oral & Maxillofacial Clinical Sciences Faculty of Medicine > Clinical Oncology Department Faculty of Medicine > Medical Microbiology Department Faculty of Medicine > Medicine Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 24 Oct 2025 07:28 |
| Last Modified: | 24 Oct 2025 07:28 |
| URI: | http://eprints.um.edu.my/id/eprint/48318 |
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