Alias, Matthew Raj and Ong, Chong-Boon and Annuar, Mohamad Suffian Mohamad (2023) Adsorption of tannase from Aspergillus ficuum to carboxyl-functionalized multi-walled carbon nanotubes. Journal of the Serbian Chemical Society, 88 (5). pp. 495-504. ISSN 0352-5139, DOI https://doi.org/10.2298/JSC221121009A.
Full text not available from this repository.Abstract
The immobilization of cross-linked tannase onto carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) was achieved via physical adsorption. Glutaraldehyde was used to cross-link the enzyme molecules. Spectroscopic and morphological characterizations of the enzyme-nanotubes composite were carried out, which authenticated the successful adsorption event. Enzyme composite is proven equal to, or even superior than free tannase, in terms of catalytic activities and stabilities, when measured under different thermal, pH and recycling conditions. Whilst both free and immobilized tannase preparations exhibited optimum catalysis at pH 5.0 and 35 degrees C, tannase-nanotubes composite possesses better thermal stability. The immobilized preparation retained 75 % of its initial catalytic activity following ten consecutive uses. The study demonstrated a facile method to produce catalytically efficient nanobiocatalyst composite for biotechnological applications.
| Item Type: | Article |
|---|---|
| Funders: | International University of Malaya-Wales [Grant No: RMC/2021-22/03] |
| Uncontrolled Keywords: | MWCNT; MWCNT-COOH; Nanobiocatalyst; Enzyme immobilization; Enzymes stability and reusability |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 06 Oct 2025 13:19 |
| Last Modified: | 06 Oct 2025 13:19 |
| URI: | http://eprints.um.edu.my/id/eprint/48280 |
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