Zulkifli, Nurfatihah and Khairat, Jasmine Elanie and Azman, Adzzie-Shazleen and Mohd Baharudin, Nur Faralyza and Malek, Nurul Adila and Zainal Abidin, Syafiq Asnawi and AbuBakar, Sazaly and Hassandarvish, Pouya (2023) Antiviral activities of streptomyces KSF 103 methanolic extracts against dengue virus type-2. Viruses-Basel, 15 (8). ISSN 1999-4915, DOI https://doi.org/10.3390/v15081773.
Full text not available from this repository.Abstract
Dengue has long been a serious health burden to the global community, especially for those living in the tropics. Despite the availability of vaccines, effective treatment for the infection is still needed and currently remains absent. In the present study, the antiviral properties of the Streptomyces sp. KSF 103 methanolic extract (Streptomyces KSF 103 ME), which consists of a number of potential antiviral compounds, were investigated against dengue virus serotype 2 (DENV-2). The effects of this extract against DENV-2 replication were determined using the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Findings from the study suggested that the Streptomyces KSF 103 ME showed maximum inhibitory properties toward the virus during the virus entry stage at concentrations of more than 12.5 mu g/mL. Minimal antiviral activities were observed at other virus replication stages; adsorption (42% reduction at 50 rho g/mL), post-adsorption (67.6% reduction at 50 mu g/mL), prophylactic treatment (68.4% and 87.7% reductions at 50 mu g/mL and 25 mu g/mL, respectively), and direct virucidal assay (48% and 56.8% reductions at 50 rho g/mL and 25 mu g/mL, respectively). The Streptomyces KSF 103 ME inhibited dengue virus replication with a 50% inhibitory concentration (IC50) value of 20.3 mu g/mL and an International System of Units (SI) value of 38.9. The Streptomyces KSF 103 ME showed potent antiviral properties against dengue virus (DENV) during the entry stage. Further studies will be needed to deduce the antiviral mechanisms of the Streptomyces KSF 103 ME against DENV.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia [Grant no. MO002-2019] |
| Uncontrolled Keywords: | Antivirals; Arbovirus; Dengue virus; Streptomyces |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 11 Oct 2025 09:12 |
| Last Modified: | 11 Oct 2025 09:12 |
| URI: | http://eprints.um.edu.my/id/eprint/48191 |
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