Assessing the bioefficacy of a commercial temephos formulation (Temebate®) for controlling Aedes albopictus larvae in different land use localities in Malaysia

Wan-Norafikah, Othman and Aliah-Diyanah, Sharifah and Atiqah-Izzah, Zaifol and Chen, Chee Dhang and Sofian-Azirun, Mohd and Lailatul-Nadhirah, Asri and Ibahim, Mohammad Johari (2023) Assessing the bioefficacy of a commercial temephos formulation (Temebate®) for controlling Aedes albopictus larvae in different land use localities in Malaysia. Experimental Parasitology, 254. ISSN 0014-4894, DOI https://doi.org/10.1016/j.exppara.2023.108627.

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Abstract

Temephos is the World Health Organization (WHO) recommended larvicide and is still being utilized worldwide to control larvae of dengue vectors; Aedes aegypti and Aedes albopictus. The efficacy of a commercial temephos product; Temebate (R) to exterminate the local populations of Ae. albopictus larvae originated from different land use particularly dengue-risk and dengue-free housing localities as well as agrarian localities including oil palm plantations, rubber estates and paddy fields was assessed to verify its bioefficacy in these localities. Field pop-ulations of Ae. albopictus larvae were attained via a larval survey at each study locality. Each Ae. albopictus larval population was subjected to a 24-h larval bioassay using Temebate (R) at operational dosage of 1 mg/L. Almost all Ae. albopictus larval populations demonstrated mortalities between 7.00% and 100.00% by the end of the first 4 h of Temebate (R) exposure with the resistance ratios between 0.94 and 8.33. After 24 h of Temebate (R) exposure, all sixteen Ae. albopictus larval populations exhibited increased mortalities with ten of them showing 100% mor-talities. These results confirmed the relevance of Temebate (R) to be continuously used by the residents of these localities as their control efforts against dengue vectors. Nevertheless, Temebate (R) application by consumers in dengue-risk localities need to be carefully monitored to prevent further development of temephos resistance among Ae. albopictus populations and substantiated with other vector control approaches.

Item Type: Article
Funders: Geran Penyelidikan Khas UiTM [Grant no. 600-RMC/GPK 5/3 (194/2020)], Institute for Medical Research, Malaysian Rubber Board, Medical Entomology Unit, UiTM Sungai Buloh Campus, Ministry of Health, Faculty of Medicine, American University of Beirut, Universiti Malaya, Universiti Teknologi MARA
Uncontrolled Keywords: Bioefficacy; Temebate (R); Commercial temephos; Aedes albopictus; Malaysia
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 22 Oct 2025 03:23
Last Modified: 22 Oct 2025 03:23
URI: http://eprints.um.edu.my/id/eprint/48118

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