Mimotopes of the Vi antigen of Salmonella enterica serovar typhi identified from phage display peptide library

Tang, Swee-Seong and Tan, Wen-Siang and Devi, Shamala and Wang, Lin Fa and Pang, Tikki and Thong, Kwai Lin (2003) Mimotopes of the Vi antigen of Salmonella enterica serovar typhi identified from phage display peptide library. Clinical and Diagnostic Laboratory Immunology, 10 (6). pp. 1078-84. ISSN 1071-412X

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Official URL: https://doi.org/10.1128/CDLI.10.6.1078-1084.2003

Abstract

The capsular polysaccharide Vi antigen (ViCPS) is an essential virulence factor and also a protective antigen of Salmonella enterica serovar Typhi. A random 12-mer phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a ViCPS-specific monoclonal antibody (MAb) ATVi. Approximately 75% of the phage clones selected in the fourth round carried the peptide sequence TSHHDSHGLHRV, and the rest of the clones harbored ENHSPVNIAHKL and other related sequences. These two sequences were also obtained in a similar panning process by using pooled sera from patients with a confirmed diagnosis of typhoid fever, suggesting they mimic immunodominant epitopes of ViCPS antigens. Binding of MAb ATVi to the mimotopes was specifically blocked by ViCPS, indicating that they interact with the same binding site (paratope) of the MAb. Data and reagents generated in this study have important implications for the development of peptide-base diagnostic tests and peptide vaccines and may also provide a better understanding of the pathogenesis of typhoid fever.

Item Type: Article
Uncontrolled Keywords: Amino Acid Sequence; Antibodies, Bacterial/blood
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 11 Mar 2011 02:36
Last Modified: 15 Oct 2018 02:36
URI: http://eprints.um.edu.my/id/eprint/478

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