Mahendra, Dedy Agoes and Yuliati, Anita and Razali, Masfueh and Abu Kasim, Noor Hayaty and Firdauzy, Muhammad Alwino Bayu and Roestamadji, Retno Indrawati and Soesilawati, Pratiwi (2025) Osteoclastogenesis markers in craniofacial bone defects after demineralized dentin material membrane implantation as guided bone regeneration. Journal of Applied Oral Science, 33. e20240254. ISSN 1678-7757, DOI https://doi.org/10.1590/1678-7757-2024-0254.
Full text not available from this repository.Abstract
Guided bone regeneration (GBR) is an alternative treatment for craniofacial bone defects reconstruction through membrane barrier adaptation, such as demineralized dentin material membrane (DDMM). DDMM is used as a substitute for GBR material, which aligns with Green Economy principles, it has a good biological osteoinductive and osteoconductive effects, and its structure resembles bones. The balance of bone remodeling when experiencing craniofacial defects will be altered and allow changes to resorption activity, so the mechanisms of osteoclastogenesis and bone resorption are vital. Objective: this article aims to analyze the expression of TNF-alpha, RANKL, and osteoclast cells count after application of DDMM as GBR in mandibular bone defects. Methodology: this is an experimental study with a post-test only control group design, which began with the randomization of 120 rats into five groups: K(-), without membrane implantation; K(+), PPCM; P1, DDMM; P2, DDMM + bone graft; P3, PPCM + bone graft. The expression of TNF-alpha, RANKL, and osteoclast cells count were observed, followed by analysis using a one-way ANOVA and post hoc Tukey HSD comparison test. Results: there were significant differences in the expression of TNF-alpha, RANKL, and osteoclast cells count in all study groups (p=0.000). TNF-alpha showed a decreasing difference with the highest expression in the K(-) group on day 3 of 12.00 +/- 2.16. RANKL expression increased on day 14 and decreased on day 21 in all groups. The osteoclast cells count generally showed a critical period with the highest increase in the K(-) group on day 14 of 73.00 +/- 0.00. Conclusion: DDMM has the potential to be a superior membrane substitute compared to PPCM as GBR in alternative treatment for craniofacial bone defects reconstruction.
| Item Type: | Article |
|---|---|
| Funders: | Universitas Airlangga International Research Network (1655/UN3.LPPM/PT.01.03/2023), Universitas Airlangga |
| Uncontrolled Keywords: | Demineralized dentin material membrane; Guided bone regeneration; TNF-alpha; RANKL; Osteoclastogenesis |
| Subjects: | R Medicine > RK Dentistry |
| Divisions: | Faculty of Dentistry > Department of Restorative Dentistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Mar 2025 05:31 |
| Last Modified: | 12 Mar 2025 05:31 |
| URI: | http://eprints.um.edu.my/id/eprint/47761 |
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