Takano, Aruto and Morinaga, Daikichi and Teramoto, Isao and Hatabu, Toshimitsu and Kido, Yasutoshi and Kaneko, Akira and Hatta, Takeshi and Tsuji, Naotoshi and Uni, Shigehiko and Sasai, Kazumi and Katoh, Hiromitsu and Matsubayashi, Makoto (2025) Detection of Eimeria oocysts in chicken feces using flotation recovery with sucrose or saturated saline solution. Acta Parasitologica, 70 (1). p. 17. ISSN 1230-2821, DOI https://doi.org/10.1007/s11686-024-00960-6.
Full text not available from this repository.Abstract
PurposeFlotation methods are widely used to detect oocysts/cysts of protozoans and eggs of helminths, except trematodes. However, details regarding the concentration and recovery rates of these parasites are poorly understood.MethodsUsing Eimeria tenella oocysts as a model parasite, the present study evaluated three check points: (1) the proportion of parasites that remain floating in flotation solution (sucrose or saturated saline) during centrifugation, (2) the proportion of oocysts that naturally float after addition of flotation solution after centrifugation, and (3) the rate of recovery on cover slips after completion of the flotation protocol.ResultsAfter centrifugation in sucrose solution and saturated saline solution, 82.4% and 60.3% of oocysts floated, respectively. After addition of flotation solution after the final centrifugation step, the recovery rates for oocysts that naturally floated again for 30 min in sucrose and saturated saline were 39.2% and 38.2%, respectively. The recovery rate on cover slips as the final step after performing a commonly used flotation method was 36.4% in sucrose solution (the rate for saturated saline solution could not be assessed due to rapid crystallization).ConclusionOur results suggest that floating oocysts could have become dispersed by the addition of flotation solution, and not all of these oocysts remained floating after an additional 30 min of settling time although collection on cover slips could be effective for accurate recovery.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science, Swedish Research Council for Health Working Life & Welfare (Forte) (21K12328), Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science |
| Uncontrolled Keywords: | Detection; Feces; Flotation method; Oocysts |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Mar 2025 07:30 |
| Last Modified: | 12 Mar 2025 07:30 |
| URI: | http://eprints.um.edu.my/id/eprint/47703 |
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