Ashokhan, Sharmilla and Karsani, Saiful Anuar and Yaacob, Jamilah Syafawati (2024) Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments. Journal of Plant Growth Regulation, 43 (9, SI). pp. 3119-3139. ISSN 0721-7595, DOI https://doi.org/10.1007/s00344-023-11065-1.
Full text not available from this repository.Abstract
Azadirachta indica or neem is recognized for its unique properties, both against insects and in improving human health. However, proteomics studies of this plant's response to abiotic stresses have yet to be reported. Application of this technique highly depends on efficient and optimized sample preparation, in deriving highly reproducible proteomics data. The present study highlights the comparison of protein extraction method which effectively recovers proteins from recalcitrant phenolic-rich tissues of neem callus. Four protocols based on TCA-acetone or/and phenol extraction methods were compared. The TCA-acetone complemented with PVPP method was the most effective approach and generated the greatest total protein yields, spot number, good resolution, and spot intensities. Subsequently, the differential proteomics performed revealed that proteins associated with oxidoreduction, energy, transcriptional, stress response, respiration, and cell division were differentially regulated in salinity stress treatment. Meanwhile, the expression of proteins classified in the group of energy, photosynthesis, protein synthesis, oxidoreduction, and post-transcriptional regulation was altered in the JA-stressed callus. The accumulation of AZA was also noticeably reduced in salinity- and JA-treated callus, suggesting these tested stress parameters were not effective in improving AZA production in the neem callus. Overall, this analysis provides valuable information on the application of plant tissue culture as an alternative for sustainable production of major bioactive constituents in A. indica. Together, the proteomics results brought forward a possible fundamental representation of the mechanism associated with salinity and JA tolerance in the neem callus.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya, Malaysia (ST009-2021), Ministry of Education, Malaysia (FP055-2017A); (FRGS/1/2017/WAB01/UM/02/2) |
| Uncontrolled Keywords: | Azadirachta indica; Abiotic stress; Salinity; Jasmonic acid; Callus; Proteomics; Azadirachtin |
| Subjects: | Q Science > QH Natural history S Agriculture > SB Plant culture |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 30 Dec 2024 04:37 |
| Last Modified: | 30 Dec 2024 04:37 |
| URI: | http://eprints.um.edu.my/id/eprint/47166 |
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