Venkatraman, Gopinath and Mohan, Priyadarshini Sakthi and Abdul-Rahman, Puteri Shafinaz and Sonsudin, Faridah and Muttiah, Barathan and Hirad, Abdurahman Hajinur and Alarfaj, Abdullah A. and Wang, Shifa (2024) Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications. Bioprocess and Biosystems Engineering, 47 (8, SI). pp. 1213-1226. ISSN 1615-7591, DOI https://doi.org/10.1007/s00449-024-02995-5.
Full text not available from this repository.Abstract
This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 mu g/mL for Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, Staphylococcus aureus than ZnO NPs at the MIC of 120 and 140 mu g/mL for Bacillus subtilis and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Furthermore, ROS detection, viability assay and bacterial membrane integrity analysis of ZnO/Ag NCs treated P. aeruginosa and S. aureus revealed the fundamental bactericidal mechanism involving cell wall, cell membrane interaction and release of cytoplasmic contents. In addition, ZnO/Ag NCs and ZnO NPs showed higher toxicity towards A549 lung cancer cells than the non-cancerous RAW264 macrophage cells, with IC50 of 242 and 398 mu g/mL respectively, compared to IC50 of 402 and 494 mu g/mL for the macrophage cells. These results suggest that the ZnO/Ag NCs can be effectively used to develop antimicrobial and anticancer materials.
| Item Type: | Article |
|---|---|
| Funders: | King Saud University (RSPD2024R677) |
| Uncontrolled Keywords: | Antibacterial; Metabolic activity; Nanocomposites; Reactive oxygen species; Cytotoxicity |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine > Department of Molecular Medicine Faculty of Science > Department of Chemistry Deputy Vice Chancellor (Research & Innovation) Office Faculty of Pharmacy > Department of Pharmaceutical Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 08 Jan 2025 08:13 |
| Last Modified: | 08 Jan 2025 08:13 |
| URI: | http://eprints.um.edu.my/id/eprint/47012 |
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