Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene.

Pathmanathan, S.G.; Cardona-Castro, N.; Sánchez-Jiménez, M.M.; Correa-Ochoa, M.M.; Puthucheary, S.D.; Thong, K.L. (2003) Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene. Journal of medical microbiology, 52 (Pt 9). pp. 773-6. ISSN 0022-2615

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Abstract

The suitability of a PCR procedure using a pair of primers targeting the hilA gene was evaluated as a means of detecting Salmonella species. A total of 33 Salmonella strains from 27 serovars and 15 non-Salmonella strains from eight different genera were included. PCR with all the Salmonella strains produced a 784 bp DNA fragment that was absent from all the non-Salmonella strains tested. The detection limit of the PCR was 100 pg with genomic DNA and 3 x 10(4) c.f.u. ml(-1) with serial dilutions of bacterial culture. An enrichment-PCR method was further developed to test the sensitivity of the hilA primers for the detection of Salmonella in faecal samples spiked with different concentrations of Salmonella choleraesuis subsp. choleraesuis serovar Typhimurium. The method described allowed the detection of Salmonella Typhimurium in faecal samples at a concentration of 3 x 10(2) c.f.u. ml(-1). In conclusion, the hilA primers are specific for Salmonella species and the PCR method presented may be suitable for the detection of Salmonella in faeces.

Item Type: Article
Creators:
  1. Pathmanathan, S.G.
  2. Cardona-Castro, N.
  3. Sánchez-Jiménez, M.M.
  4. Correa-Ochoa, M.M.
  5. Puthucheary, S.D.
  6. Thong, K.L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
Journal or Publication Title: Journal of medical microbiology
Additional Information: Institute of Postgraduate Studies, Department of Medical Microbiology, Faculty of Medicine and Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
Uncontrolled Keywords: Animals; Bacterial Proteins/genetics; DNA Primers;
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 11 Mar 2011 09:57
Last Modified: 20 Oct 2014 15:54
URI: http://eprints.um.edu.my/id/eprint/470

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