Krishnan, Illayaraja and Chan, Alvin Man Lung and Law, Jia Xian and Ng, Min Hwei and Jayapalan, Jaime Jacqueline and Lokanathan, Yogeswaran (2024) Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review. International Journal of Molecular Sciences, 25 (10). p. 5340. ISSN 1661-6596, DOI https://doi.org/10.3390/ijms25105340.
Full text not available from this repository.Abstract
Numerous challenges remain within conventional cell-based therapy despite the growing trend of stem cells used to treat various life-debilitating diseases. These limitations include batch-to-batch heterogeneity, induced alloreactivity, cell survival and integration, poor scalability, and high cost of treatment, thus hindering successful translation from lab to bedside. However, recent pioneering technology has enabled the isolation and enrichment of small extracellular vesicles (EVs), canonically known as exosomes. EVs are described as a membrane-enclosed cargo of functional biomolecules not limited to lipids, nucleic acid, and proteins. Interestingly, studies have correlated the biological role of MSC-EVs to the paracrine activity of MSCs. This key evidence has led to rigorous studies on MSC-EVs as an acellular alternative. Using EVs as a therapy was proposed as a model leading to improvements through increased safety; enhanced bioavailability due to size and permeability; reduced heterogeneity by selective and quantifiable properties; and prolonged shelf-life via long-term freezing or lyophilization. Yet, the identity and potency of EVs are still relatively unknown due to various methods of preparation and to qualify the final product. This is reflected by the absence of regulatory strategies overseeing manufacturing, quality control, clinical implementation, and product registration. In this review, the authors review the various production processes and the proteomic profile of MSC-EVs.
| Item Type: | Article |
|---|---|
| Funders: | Faculty of Medicine, Universiti Kebangsaan Malaysia |
| Uncontrolled Keywords: | extracellular vesicle; exosome; mesenchymal stem cell; isolation; enrichment; proteomic analysis |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Department of Molecular Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 13 Jan 2025 01:17 |
| Last Modified: | 13 Jan 2025 01:17 |
| URI: | http://eprints.um.edu.my/id/eprint/46959 |
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