Putra, Sharifah Shahira Syed and Chew, Chia Yong and Hayyan, Adeeb and Elgharbawy, Amal A. M. and Taskin-Tok, Tugba and Hayyan, Maan and Ngoh, Gek Cheng and Saleh, Jehad and Al Abdulmonem, Waleed and Alghsham, Ruqaih S. and Nor, Mohd Roslan Mohd and Aldaihani, Ahmad G. H. and Basirun, Wan Jefrey (2024) Nanodiamonds and natural deep eutectic solvents as potential carriers for lipase. International Journal of Biological Macromolecules, 270 (2). p. 132245. ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2024.132245.
Full text not available from this repository.Abstract
This study investigates the use of nanodiamonds (ND) as a promising carrier for enzyme immobilization and compares the effectiveness of immobilized and native enzymes. Three different enzyme types were tested, of which Rhizopus niveus lipase (RNL) exhibited the highest relative activity, up to 350 %. Under optimized conditions (1 h, pH 7.0, 40 degrees C), the immobilized ND-RNL showed a maximum specific activity of 0.765 U mg(-1), significantly higher than native RNL (0.505 U mg(-1)). This study highlights a notable enhancement in immobilized lipase; furthermore, the enzyme can be recycled in the presence of a natural deep eutectic solvent (NADES), retaining 76 % of its initial activity. This aids in preserving the native conformation of the protein throughout the reusability process. A test on brine shrimp revealed that even at low concentrations, ND-RNL had minimal toxicity, indicating its low cytotoxicity. The in silico molecular dynamics simulations performed in this study offer valuable insights into the mechanism of interactions between RNL and ND, demonstrating that RNL immobilization onto NDs enhances its efficiency and stability. All told, these findings highlight the immense potential of ND-immobilized RNL as an excellent candidate for biological applications and showcase the promise of further research in this field.
| Item Type: | Article |
|---|---|
| Funders: | Sustainable Process Engineering Centre (SPEC), Ministry of Higher Education, Research and Innovation (MoHERI) Oman through TRC (BFP/RGP/EBR/22/378), International Institute for Halal Research and Training (INHART), Faculty of Engineering, University of Malaya, International Islamic University Malaysia, RU Geran-Fakulti Program University Malaya (GPF015A-2023) |
| Uncontrolled Keywords: | Rhizopus niveus lipase; Natural deep eutectic solvents; Molecular dynamics |
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology |
| Divisions: | Academy of Islamic Studies Faculty of Engineering Faculty of Engineering > Department of Chemical Engineering Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Jan 2025 00:54 |
| Last Modified: | 14 Jan 2025 00:54 |
| URI: | http://eprints.um.edu.my/id/eprint/46935 |
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