Zambry, Nor Syafirah and Awang, Mohd Syafiq and Hamzah, Hairul Hisham and Mohamad, Ahmad Najib and Khalid, Muhammad Fazli and Khim, Beh Khi and Bustami, Yazmin and Jamaluddin, Nurul Fauzani and Ibrahim, Fatimah and Aziah, Ismail and Abd Manaf, Asrulnizam (2024) A portable label-free electrochemical DNA biosensor for rapid detection of Salmonella Typhi. Analytical Methods, 16 (30). pp. 5254-5262. ISSN 1759-9660, DOI https://doi.org/10.1039/d4ay00888j.
Full text not available from this repository.Abstract
A highly accurate, rapid, portable, and robust platform for detecting Salmonella enterica serovar Typhi (S. Typhi) is crucial for early-stage diagnosis of typhoid to avert and control the outbreaks of this pathogen, which threaten global public health. This study presents a proof-of-concept for our developed label-free electrochemical DNA biosensor system for S. Typhi detection, which employs a printed circuit board gold electrode (PCBGE), integrated with a portable potentiostat reader. Initially, the functionalized DNA biosensor and target detection were characterized using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) methods using a benchtop potentiostat. Interestingly, the newly developed DNA biosensor can identify target single-stranded DNA concentrations ranging from 10 nM to 20 mu M, achieving a detection limit of 7.6 nM within a brief 5 minute timeframe. Under optimal detection conditions, the DNA biosensor exhibits remarkable selectivity, capable of distinguishing a single mismatch base pair from the target single-stranded DNA sequence. We then evaluated the feasibility of the developed DNA biosensor system as a diagnostic tool by detecting S. Typhi in 50 clinical samples using a portable potentiostat reader based on the DPV technique. Remarkably, the developed biosensor can distinctly distinguish between positive and negative samples, indicating that the miniaturised DNA biosensor system is practical for detecting S. Typhi in real biological samples. The developed DNA biosensor device in this work proves to be a promising point-of-care (POC) device for Salmonella detection due to its swift detection time, uncomplicated design, and streamlined workflow detection system.
| Item Type: | Article |
|---|---|
| Funders: | Higher Institution Centre of Excellence (HICoE) from the Ministry of Higher Education, Malaysia (311/CIPPM/4401005), Collaborative Research in Engineering, Science, and Technology (CREST) (304/CIPPM/6150181/C121), Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Malaysia (TDF07211415 (MOSTI002B-2022TED1)) |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Engineering Faculty of Engineering > Biomedical Engineering Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 25 Mar 2025 03:27 |
| Last Modified: | 25 Mar 2025 03:27 |
| URI: | http://eprints.um.edu.my/id/eprint/46826 |
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