Yew, Jia Sheng and Ong, Seng-Kai and Lim, Hui Xuan and Tan, Soon Hao and Ong, Kien Chai and Wong, Kum Thong and Poh, Chit Laa (2024) Immunogenicity of trivalent DNA vaccine candidate encapsulated in Chitosan-TPP nanoparticles against EV-A71 and CV-A16. Nanomedicine, 19 (21-22). pp. 1779-1799. ISSN 1743-5889, DOI https://doi.org/10.1080/17435889.2024.2372243.
Full text not available from this repository.Abstract
Aim: To develop a trivalent DNA vaccine candidate encapsulated in Chitosan-TPP nanoparticles against hand foot and mouth disease (HFMD) and assess its immunogenicity in mice. Materials & methods: Trivalent plasmid carrying the VP1 and VP2 genes of EV-A71, VP1 gene of CV-A16 was encapsulated in Chitosan-TPP nanoparticles through ionic gelation. In vitro characterization and in vivo immunization studies of the CS-TPP-NPs (pIRES-VP121) were performed. Results: Mice administered with CS-TPP NPs (pIRES-VP121) intramuscularly were observed to have the highest IFN-gamma response. Sera from mice immunized with the naked pDNA and CS-TPP-NPs (pIRES-VP121) demonstrated good viral clearance against wild-type EV-A71 and CV-A16 in RD cells. Conclusion: CS-TPP-NPs (pIRES-VP121) could serve as a prototype for future development of multivalent HFMD DNA vaccine candidates.
| Item Type: | Article |
|---|---|
| Funders: | Sunway University International Network Grant Scheme (STR-IRNGS-SMLS-CVVR-01-2021) |
| Uncontrolled Keywords: | DNA; immunology/infectious diseases; nanoparticles; vaccines; viruses |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Biomedical Science Department Faculty of Medicine > Pathology Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 26 Mar 2025 08:25 |
| Last Modified: | 26 Mar 2025 08:25 |
| URI: | http://eprints.um.edu.my/id/eprint/46805 |
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