Tan, Yen Yee and Abdullah, Dalia and Abu Kasim, Noor Hayaty and Yazid, Farinawati and Apandi, Nurul Inaas Mahamad and Ramanathan, Anand and Soo, Eason and Radzi, Rozanaliza and Teh, Lay Ann (2024) Histological characterization of pulp regeneration using decellularized human dental pulp and mesenchymal stem cells in a feline model. Tissue and Cell, 90. p. 102484. ISSN 0040-8166, DOI https://doi.org/10.1016/j.tice.2024.102484.
Full text not available from this repository.Abstract
Regenerative endodontics aims to restore pulp tissues, thus preserving the vitality of the tooth. One promising approach involves the utilization of decellularized human dental pulp (DHDP) as a scaffold repopulated with Wharton's Jelly mesenchymal stem cells (WJMSCs). This study aimed to regenerate pulp tissues using DHDP and WJMSCs following pulpectomy in mature canine teeth of a feline animal model and to investigate the histological features of the regenerated pulp. A 12-month-old male domestic shorthaired felines were used as subjects. Teeth were categorized into untreated (Group 1), pulpectomy with mineral trioxide aggregate (MTA) (Group 2), and pulpectomy with DHDP-repopulated scaffold and MTA (Group 3). The animals were sacrificed six weeks postintervention. H&E and immunohistochemistry using anti-collagen type 1 and laminin antibodies were used to stain the tissue sections. Histological examinations presented pulp-like tissues in Group 3, with tissue components similar to the structures found in Group 1. Immunohistochemical analysis demonstrated the presence of collagen type I and laminin within the regenerated tissues. The root canals of teeth in Group 2 were devoid of pulpal tissue. DHDP with WJMSCs can potentially be used for pulp regeneration, supporting the modality for developing new clinical protocols in stem cell therapy.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education Malaysia (MOHE) through Fundamental Research Grant Scheme (FRGS/1/2022/SKK11/UKM/01/3) |
| Uncontrolled Keywords: | Anti-collagen type 1; Immunohistochemistry; Laminin; Regenerative endodontics; Wharton's Jelly Mesenchymal Stem Cells |
| Subjects: | R Medicine > RK Dentistry |
| Divisions: | Faculty of Dentistry Faculty of Dentistry > Dept of Oral & Maxillofacial Surgery Faculty of Dentistry > Department of Restorative Dentistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 27 Mar 2025 07:06 |
| Last Modified: | 27 Mar 2025 07:06 |
| URI: | http://eprints.um.edu.my/id/eprint/46775 |
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