Genomic variability within laboratory and wild isolates of the trichostrongyle mouse nematode heligmosomoides polygyrus

Abu-Madi, M.A. and Reid, A.P. and Lewis, J.W. and Mohd-Zain, S.N. (2000) Genomic variability within laboratory and wild isolates of the trichostrongyle mouse nematode heligmosomoides polygyrus. Journal of Helminthology, 74 (3). pp. 195-201. ISSN 0022-149X

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Abstract

PCR-RFLP techniques have been used to characterize wild and laboratory isolates of the trichostrongyle nematode Heligmosomoides polygyrus from the wood mouse Apodemus sylvaticus and the laboratory mouse Mus musculus respectively. Both isolates can be distinguished by eight endonuclease digestions of the ITS region of the rDNA repeat namely, Alu I, Dde I, Hpa II, Hae III, Hinf I, Hha I, Pvu II and Sal I. In two of the digests, Hinf I and Rsa I, a minor polymorphism was observed in the wild isolate of H. polygyrus which has been cultured in laboratory-bred A. sylvaticus for several generations when compared with H. p. polygyrus from wild A. sylvaticus. A minor polymorphism was also identified in further wild isolates of H. polygyrus collected from A. sylvaticus in a field site in Egham, Surrey. However no evidence of polymorphism was observed in the laboratory isolate of H. polygyrus from the CD1 strain of M. musculus and the laboratory-bred A. sylvaticus. Reasons for this are discussed and further studies on the population genetics of H. polygyrus are suggested.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Polymerase chain-reaction, Parasitic nematodes, Wood mouse, Nematospiroides-dubius, Species identification, Dna, Trichinella, Populations, Ecology, Rodents
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 05 Feb 2013 03:43
Last Modified: 11 Nov 2013 02:11
URI: http://eprints.um.edu.my/id/eprint/4673

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