Du, Shengrong and Huynh, Tony and Lu, Yen-Zhen and Parker, Bradyn J. and Tham, Stephen K. and Thissen, Helmut and Martino, Mikael M. and Cameron, Neil R. (2024) Bioactive polymer composite scaffolds fabricated from 3D printed negative molds enable bone formation and vascularization. Acta Biomaterialia, 186. pp. 260-274. ISSN 1742-7061, DOI https://doi.org/10.1016/j.actbio.2024.07.038.
Full text not available from this repository.Abstract
Scaffolds for bone defect treatment should ideally support vascularization and promote bone formation, to facilitate the translation into biomedical device applications. This study presents a novel approach utilizing 3D-printed water-dissolvable polyvinyl alcohol (PVA) sacrificial molds to engineer polymerized High Internal Phase Emulsion (polyHIPE) scaffolds with microchannels and distinct multiscale porosity. Two sacrificial mold variants (250 mu m and 500 mu m) were generated using fused deposition modeling, filled with HIPE, and subsequently dissolved to create polyHIPE scaffolds containing microchannels. In vitro assessments demonstrated significant enhancement in cell infiltration, proliferation, and osteogenic differentiation, underscoring the favorable impact of microchannels on cell behavior. High loading efficiency and controlled release of the osteogenic factor BMP-2 were achieved, with microchannels facilitating release of the growth factor. Evaluation in a mouse critical-size calvarial defect model revealed enhanced vascularization and bone formation in microchanneled scaffolds containing BMP-2. This study not only introduces an accessible method for creating multiscale porosity in polyHIPE scaffolds but also emphasizes its capability to enhance cellular infiltration, controlled growth factor release, and in vivo performance. The findings suggest promising applications in bone tissue engineering and regenerative medicine, and are expected to facilitate the translation of this type of biomaterial scaffold.
| Item Type: | Article |
|---|---|
| Funders: | Monash Animal Research Platform, Monash Histology Platform, Sylvia and Charles Viertel Charitable Foundation, State Government of Victoria, Department of Chemical Engineering, Monash University, Australian Research Council for the Industrial Transformation Training Centre for Cell and Tissue Engineering Technologies, National Health and Medical Research Council, National Health and Medical Research Council |
| Uncontrolled Keywords: | PolyHIPEs; 3D printing; Microchannels; Vascularization; Bone regeneration |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 22 Oct 2025 01:33 |
| Last Modified: | 22 Oct 2025 01:33 |
| URI: | http://eprints.um.edu.my/id/eprint/46495 |
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