Kasim, Noraini and Afzan, Adlin and Mediani, Ahmed and Low, Kah Hin and Ali, Abdul Manaf and Mat, Nashriyah and Wolfender, Jean-Luc and Ismail, Nor Hadiani (2022) Correlation of chemical profiles obtained from 1H-NMR and LC-MS metabolomics with α-glucosidase inhibition activity for varietal selections of Ficus deltoidea. PHYTOCHEMICAL ANALYSIS, 33 (8). pp. 1235-1245. ISSN 1099-1565, DOI https://doi.org/10.1002/pca.3175.
Full text not available from this repository.Abstract
Introduction Ficus deltoidea Jack (Moraceae) is a plant used in Malaysia to treat various ailments, including diabetes. The presence of several varieties raises essential questions regarding which is the potential bioactive variety and what are the bioactive metabolites. Objectives Here, we explored the phytochemical diversity of the seven varieties from Peninsular Malaysia using Nuclear Magnetic Resonance (NMR) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses and correlated it with the alpha-glucosidase inhibitory activity. Methodology The Nuclear Overhauser Effect Spectroscopy (NOESY) One-Dimensional (1D)-NMR and LC-MS data were processed, annotated, and correlated with in vitro alpha-glucosidase inhibitory using multivariate data analysis. Results The alpha-glucosidase results demonstrated that different varieties have varying inhibitory effects, with the highest inhibition rate being F. deltoidea var. trengganuensis and var. kunstleri. Furthermore, diverse habitats and plant ages could also influence the inhibitory rate. The heat map from NMR and LC-MS profiles showed unique patterns according to varying levels of alpha-glucosidase inhibition rate. The Partial Least Squares (PLS) model constructed from both NMR and LC-MS further confirmed the correlation between the alpha-glucosidase inhibition rate of F. deltoidea varieties and its metabolite profiles. The Variable Influence on Projection (VIP) and correlation coefficient (p(corr)) values values were used to determine the highly relevant metabolites for explaining the anticipated inhibitory action. Conclusion NMR and LC-MS annotations allow the identification of flavan-3-ols and proanthocyanidins as the key bioactive factors. Our current results demonstrated the value of multivariate data analysis to predict the quality of herbal materials from both biological and chemical aspects.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Agriculture and Food Industries (Malaysia) through NKEA Agriculture EPP, Ministry of Agriculture and Food Industries (Malaysia) through NKEA Agriculture EPP [NH0513D018], Ministry of Agriculture and Food Industries (Malaysia) through NKEA Agriculture EPP [100-RMI/MOA 16/6/2 [2/2013]] |
| Uncontrolled Keywords: | bioactive metabolites; bioactive varieties; Ficus deltoidea varieties; multivariate data analysis; potential antidiabetic; quality control |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms Koh Ai Peng |
| Date Deposited: | 25 Jul 2024 01:32 |
| Last Modified: | 25 Jul 2024 01:32 |
| URI: | http://eprints.um.edu.my/id/eprint/46245 |
Actions (login required)
 |
View Item |
